Effect Of Amino Acids Composition And Carbohydrates Constitute On Mixed Rumen Fermentation In Vitro

In this study,two laoshan dairy goat with permanent rumen fistula used to be donor. Pure amino acid ,starch , and cellulose used to be substrate. There are three amino acid groups: equinolar group (amino acid equimolar mixture), microbial group (refer to microbial-amino acid ),casein group(refer to casein-amino acid ). Use the artificial rumen in vitro to study the amino acids in different proportions under the influence on rumen fermentation in vitro, the test is divided into three part. Part I: Pure Amino Acids as sole substrate of fermentation in vitro culture conditions, constitute a specific EAA rumen fermentation. Part II: A fixed ratio of amino acids, adding different proportions of starch and cellulose, starch and cellulose observed different proportions of rumen fermentation in vitro. Part III: Adding carbohydrates in the different ratio of amino acids under the conditions of adding the mixture of rumen fermentation in vitro. The results show that:1. Effect of specific EAA composition on mixed rumen fermentation in vitroCulture medium pH values varied between 6.87 6.96, the pH value of microbial group had the highest mean was 6.94, the molar group had the minimum mean was 6.91 for microbial fermentation (pH 5.5 7.5). Medium NH₃-N concentration varied between 3.54 9.50mg/100ml, the highest average of microbial group is 6.11mg/100ml, so molar was lowest for the 5.62mg/100ml, over time, NH₃-N concentration showed a gradually increasing trend; The TVFA concentration of each treatment group , acetic acid, propionic acid, butyric acid, the ratio of total volatile fatty acids were not significantly different; The proportion of each treatment group in the substrate amino acid composition of different bacteria on the fermentation broth significant influence protein concentration, and other fermented liquid mole group of bacterial proteins was significantly higher than the microbial groups and the casein group, the mean value is 3.5993mg/ml, the protein yield of microbial bacteria group and the casein group was not significant.2. Effect of carbohydrate type on mixed rumen fermentation in vitroCulture medium pH values of between 6.08 6.90, the mean pH value of A (starch: cellulose 0:100) group of up to 6.85, D (starch: cellulose 100:0) were the lowest for the 6.48 for Microbiology fermentation (pH 5.5 7.5), with the substrate to increase the starch content, pH value decreased. Medium ammonia concentration varied between 3.38 9.94mg/100ml, mean to A (starch: cellulose 0:100) was the highest for the 7.51mg/100ml, (of starch: cellulose to 100:0) were the lowest was 6.54 mg/100ml, over time, ammonia concentration showed a gradually increasing trend, with the increase of starch content in the substrate, the fermentation liquid ammonia nitrogen concentration decreased; each treatment group TVFA concentration of propionic acid, butyric acid by the ratio of total volatile fatty acids were significantly different (P <0.05); the substrate treated with the composition ratio of starch and cellulose fermentation of different cell protein concentration on the impact of significant differences (P <0.05), with the starch substrate content increased, fermentation by protein production was increased.3. The mixed interaction of EAA coposition and carbohydrate type on mixed rumen fermentation in vitro.In this study, through the use of pure amino acids, starch and cellulose as a fermentation substrate, by limiting the fermentation substrate ratio of starch and cellulose, amino acid composition of different amino acids and carbohydrates on in vitro culture conditions rumen microorganisms and fermentation characteristics. To two to install a permanent rumen fistula in the Laoshan dairy goat rumen fluid as a donor. Substrate divided into: different ratio of starch and cellulose, and mixtures of different amino acid composition. The results show that: in vitro culture conditions, substrate ratio and amino acid composition of different carbohydrates for rumen fermentation and microbial growth have certain influence. Culture medium pH values varied between 6.09 6.93 for microbial fermentation (pH 5.5 7.5). Medium ammonia concentration between 2.20 8.14mg/100ml change over time, ammonia concentration showed a gradually increasing trend (P <0.05), but at different time points ammonia concentration in the tolerance range of microbial growth ; each treatment group TVFA concentration, acetic acid, propionic acid, butyric acid by the ratio of total volatile fatty acids were not significantly different; the substrate treated with starch and cellulose patients with different protein on rumen protozoa and significant effect on bacterial protein concentration (P <0.05), amino acid composition of different protozoa on rumen protein and bacterial protein had no significant effect.