| This study was conducted on Pelteobagrus fulvidraco to study the effects of different contents of konjac mannanoligosaccharides on growth performance and digestive enzyme activity, non-specific immune function, biochemical characteristic, and intestinal flora. The experimental fish were divided into five groups. The control group(C) supplemented with basal diet, the treatment groups (KM0.1, KM0.2, KM0.3) were contained 0.1%,0.2%, 0.3% konjac mannanoligosaccharides (Kon-Mos), the positive control group (M0.3) were fed diets with 0.3% Yeast cell wall mannanoligosaccharides (Mos). The results showed that:1. Fish fed diets with Kon-Mos supplementation for 49 d, had significantly higer relative gain rate, viscera index and lower feed conversion ratio. The relative gain rate in group KM0.2 was highest (95.68%), and feed conversion ratio was lowest (1.63), and significantly lower than the M0.3 group. In our current study, we tested whether the addition of Kon-Mos would effects the digestive enzyme activities of yellow catfish. Fish fed diet containing different levels of Kon-Mos and 0.3% Yeast Mos had greater (P<0.05) protease, lipase and amylase activities in the intestine and hepatopancreas than those fed the basal level of diet. The results presented here show that a supplement.of 0.2 % Kon-Mos diet significantly increased the digestive enzyme activities.2. Between 14 and 28 day, the phagocytic activity of leukocytes in group KM0.2 were significantly higher than the control group (P<0.05), phagocytic percentage (PP) and phagocytic index (PI) reached to the top level on the 14 d, respectively 66.00%,5.24. The phagocytic activity of leukocytes in group KM0.3, M0.3 were not significantly higher than the control group (P>0.05).The lysozyme activity (LZM), blood serum superoxide dismutase activity (SOD) in group KM0.2 were significantly higher than the control group (P<0.05), LZM reached to the top level on the 35 d, SOD activity reached to the top level on the 7 d, respectively 0.31,136.67. The blood serum SOD activity in group KM0.3, M0.3 were not significantly higher than the control group.3. In the present study, from 28 to 35 d the blood glucose content in group KM0.2 and KM0.3 were significantly lower than those fed the basal diet (P<0.05), but throughout the course of the experiment, M0.3 were not significantly effect on blood glucose content (P>0.05); Blood serum glutamic-oxaloacetic transaminase(GOT), glutamic-pyruvic transaminase (GPT) activity were increased trend in fish fed the Kon-Mos and yeast Mos than control, but effect was not significantly; From 28 to 35 d, there were remarkable effect of blood serum alkaline phosphatase (ALP) activity supplemented with treatment diets (P<0.05), and ALP activity was significantly increased by dietary 0.2% Kon-Mos on the experiment from 14 to 35 d; Blood sreum the levels of total cholesterol (CHO) and triglycerides (TG) were lower by dietary treatments than the control, and effects of KM0.2 group were significantly (P<0.05); Fish fed with treatment diets, contents of blood serum total protein (TP) and Calcium (Ca2+) have increased trend, and KM0.2 significantly increased than those fish fed basal diet (P<0.05).4. The fish fed with diffent Kon-Mos and 0.3% yeast Mos for 49 days, the quantities of Bifidobacterium in the foregut, midgut, and hindgut were higher than control, and the quantities of Bifidobacterium showed a decreasing trend after the first rise in KM0.2 diet. During the experiment, the quantities of Bifidobacterium in group KM0.2 were significant higher than the control group (P<0.05), in group M0.3 were not significantly higher than the control group.The Pelteobagrus fulvidraco fed with diffent levels of Kon-Mos, the quantities of Escherihia coli and Aeromonas were decreased, there was an increasing trend after the first decrease in KM0.2 diet for the quantities of Escherihia coli and Aeromonas in intestinal canal, with the increase in the amount of Kon-Mos added, during the experiment, in group KM0.2 were significantly lower than the control group (P<0.05), in group M0.3 were not significantly lower than the control group. |
