Synthesis And Antioxidant Activity Of DMY Derivatives

Dihydromyricetim (DMY) has conspicuous physiological efficiency and antioxidant activity, which enriched in ampelosis grossedetala. Current research focuses on how to improve its effect and extend its application range.There were two research approach of the structure modification with DMY. Based on prodrug principle, six hydroxyl were protected by acetyl, and then release the active base, so it was stable in vitro and variable in vivo. Secondly, DMY and gallic acid were used to synthesize DMY-gallic acid ester which structure likes EGCG. Moreover, DMY and its derivatives’structure-activity relationship were checked by oxidation resistance test. The research results of this topic were as follows:1. DMY-1、DMY-2、DMY-3、DMY-4 were the products of DMY acetylation study.They were tested by IR、NMR、MS. They were sex-acetylated、tetra-acetylated、penta-acetylated and mon-acetylated derivatives.2. DMY-5 and DMY-6 were formed during the synthesis Process of DMY-gallic acid ester. They were tested by NMR and MS, the results showed that DMY-6 was addition compound of DMY and acetone, DMY-6’s undetermined.3. The solubility study results showed that derivatives’dissolubility in water and EtOH were decreased but increased in Ethyl Acetate.4. The antioxidant activity of DMY and its derivatives were explored with DPPH·、O2-·and·OH. All of them have anti-oxidative capacity. DMY’s effect was more than others.