Study On Evaluation Method Of Radix Aconiti Lateralis Preparata Substance Quality Based On Efficacy

Objective: To establish the system of authentic curative effect of fine quality evaluation method of famous-region drug monkshood in Sichuan province. What the" famous-region drug " means strong, with a long history and culture quality, producing area is moderate, rich production, and recovery processing, curative effect is distinct, with unique geographical features of medicinal herbs. It is an important method to distinguish authenticity, quality control of traditional Chinese medicine. The nature of its region refers to the efficacy of medicine truly authentic, curative effect is distinct, exact and reliable. At present the quality evaluation of Chinese drugs method mainly includes:exterior morphology, chemical composition, genetic, biological activity and other indicators. But the integrity of the Chinese quality evaluation system has not yet been established as the lack of affect the quality of Chinese internal and external factors affecting, finally discuss clinical curative effect of the combination and an ingredient correlation analysis among the "genes shape-bad about a biological effect". So, this paper will choose monkshood as the research object from the appearance shape, genetic, indicator elements, trace elements, such as biological activity index of lateral root from different points of authentic sex is to discuss the curative effect of fine quality, the authentic evaluation method study, attempting to establish efficacy as the core of authentic assessment method of lateral root system.Methods and results1. Research on genetic indicatorsBy PCR assay to detect DNA sequence different origin of the medicinal materials of lateral amplification products. It removes the primer area by CodonCode Aligner V 3.7.1 proofreading. To compare the all sequence with MEGA5.0 software analysis. Results show that the Shanxi hanzhong’s samples without lateral root of aconite psbA-trnH and other selected samples of three psbA-trnH sequence fragment length 317 bp, sequence are exactly the same, no mutation loci.2. Research on exterior shape indexThe aconite is measured its length, width and thickness by vernier calipers and meters external and internal color with Color difference meter. The results showed that there is significant difference (P< 0.01, P< 0.01, P< 0.05) among yunnan luquan, shanxi hanzhong and sichuan jiangyou,liangshan of the aconite root of length, width, thickness, weight. And with the sichuan jiangyou monkshood processed products producing black along the piece slightly larger, heavier; Black shun-the medicinal materials of external color-of shanxi hanzhong shallow lateral root of aconite, and have significant difference compared with sichuan jiangyou; Medicinal powder-the internal color of the medicinal materials-in sichuan jiangyou is darkest, have significant difference (P< 0.01, P< 0.01, P< 0.05) compared with liangshan in sichuan, shanxi hanzhong, yunnan luquan.3. Research on trace element indexReference to "Chinese pharmacopoeia" 2010 edition a appendix XI D icp-ms method for determining the different regions of the black of lateral shun in copper, iron, manganese, lead, cadmium, zinc, calcium, chromium, nickel, magnesium content of 10 kinds of trace elements. Results show that besides cadmium, different regions of the black of lateral suitable in other trace elements have significant difference, and there is no doubt about the importance of trace elements for the medicinal efficacy.4. Research on chemical component indexReference to "Chinese pharmacopoeia" 2010 version of a content determination methods for determining different lateral root of aconite origin black smooth piece of lateral root of aconite and extract content of total alkaloids and ester alkaloids. Results show that the different region of the black of lateral arranges in the total alkaloid content is between 0.20%-0.45%, and it is consistent with the literature[12]. A double ester type alkaloid and single ester type alkaloid content are all meet the requirements of pharmacopoeia content; the alkaloid content of black suitable pieces of aqueous extract have reduced compared with the decoction pieces; Aconite alkaloid content in alcohol extract compared with the slices, total alkaloid content increases, the double ester type alkaloid, single ester type alkaloid content is lower.5. Research on biological activity evaluation index of lateral root of aconiteAccording to this study choices three classic clinical applications of aconite back to Yang save inverse effect and the clinical curative effect in cardiovascular system, selection of pharmacological effects associated with efficacy of lateral attending-shock resistance, resistance to slow arrhythmia, treatment of heart failure in study on biological activity evaluation of clinical application.Poison to death in mice within the protective effects of observation of different origin of the aqueous extract of lateral (7.5 g/kg) of anti-shock effect; With verapamil injection cause bradycardia model in rats, observe the different origin of the alcohol extract of lateral (5 g/kg) for the period between arrhythmia animal HR, P-R, P wave, QRS wave index change, explore its action mechanism; Make mould with pentobarbital sodium in acute heart failure rat model, each take delivery before the indicators and 16 HR recor synchronous record, left ventricular pressure (LVSP), left ventricular pressure rise rate (+dp/dtmax), left ventricular pressure drop rate (-dp/dtmin), dynamic observation of different origin of 急性心衰 rats hemodynamic effects of lateral root of aconite, and big abdomen aorta in determination of blood plasma arginine vasopressin (AVP), endothelia 1 (ET 1), sodium heart element (ANP), angiotensin (A Ⅱ) change and the level of brain natriuretic factor (BNP) synthesis and expression of gene.The results showed that:Different regions of the water extract of lateral (7.5 g/kg) can obviously reduce the mice induced by endotoxin attack deaths, of which the most sichuan jiangyou of the role of lateral obvious; Jiangyou, sichuan aconite alcohol extract (5 g/kg) of rats caused by verapamil injection duration between HR decrease, P-R, P wave amplitude reduced, wide QRS wave abnormalities with the improvement of the comprehensive function, electrocardiogram (ecg) close to the normal control group; Yunnan luquan monkshood alcohol extract (5 g/kg) improved HR, restore between P and R period, improve the effect of QRS wave anomalies; Liangshan, sichuan aconite root and hanzhong city in shaanxi province of the alcohol extract of lateral (5 g/kg) improved HR, restore P-R interphase effect, but the abnormality of the P wave, QRS wave effect is not obvious; Dynasties at the same time, the group can improve the hemodynamics of acute heart failure rats of indicators, to speed up the heart rate, increased left ventricular pressure, improve the maximum increase rate and the maximum decrease rate of the left ventricle and authentic jiangyou effect is most obvious lateral root of aconite. Its mechanism and its mechanism of action and the plasma level of AVP content, inhibit the BNP gene synthesis in ventricular muscle and expression, and shrinkage vascular factor ET-1, A Ⅱ level and ANP gene expression. Biological activity evaluation results showed that different regions of the processed products of lateral, the tradition of the original region of lateral jiangyou, sichuan aconite clinical curative effect, the most obvious is obviously better than the strongest bioactivity, liangshan, sichuan, yunnan luquan, shaanxi hanzhong aconite root.Conclusion:Traditional appearance shape, DNA sequencing, pharmacopoeia identified the study of the chemical composition, trace elements are difficult to clarify medicinal herbs on the clinical curative effect and cannot directly reflect the essence of medicinal efficacy of fine quality of lateral root of aconite. But there are some influences on building of medicinal materials authentic curative effect of fine quality, "gene-appearance form-chemical-biotic environment-biological effect" the authentic of the overall evaluation system should be through a lot of experiment again; Intensity of biological activity is can directly reflect the nature of the good efficacy of medicine-the clinical curative effect is distinct is the core of authentic assessment method of lateral index, there is a great significance to evaluate the quality of medicinal materials. Therefore, it is necessary to establish the comprehensive evaluation system of influence factors and the curative effect of combination, with efficacy (biological activity) as the core, guaranteeing the authentic and the feature of Chinese drugs.