| Objective To measure the expression levels of lnc RNA CTBP1-AS and SRA in PCOS patients and healthy control women, and analyze the correlation between CTBP1-AS, SRA and the disease as well as multiple key endocrine parameters of PCOS. We speculate that CTBP1-AS and SRA may be involved in the development of PCOS.Methods We recruited 23 patients with PCOS and 17 matched healthy women before entering in vitro fertilization(IVF) programme due to male factor infertility. First, we measured the expression of CTBP1-AS and SRA in the peripheral blood leukocytes in PCOS patients and healthy control women by q RT-PCR. Second, the correlation between CTBP1-AS, SRA and the disease as well as multiple key endocrine parameters of PCOS was analyzed by using multiple statistical methods.Results 1. CTBP1-AS expression level in peripheral blood leukocytes was significantly higher in the women with PCOS than that in the controls after age and BMI adjustment(-ln CTBP1-AS, 5.323±0.205 vs. 2.059±0.210, P<0.05).2. Individuals having higher CTBP1-AS expression had significantly greater disease risk than those having lower expression(OR=1.822, 95% CI = 1.223–2.225, P<0.05). We also identified CTBP1-AS expression as an independent risk factor for PCOS.3. A positive correlation was observed between the CTBP1-AS expression and the total testosterone(TT) concentration either unadjusted or after adjusted for age, BMI and HOMA-IR(r = 0.453, P<0.05).4. Lnc RNA SRA expression in peripheral blood leukocytes was significantly higher in the women with PCOS than that in the controls(5.95×10-2 [3.54×10-3-8.41×10-1] vs. 9.89×10-3 [5.97×10-4-1.42×10-1], respectively, P < 0.05) after age and BMI adjustment.5. In PCOS group, there was a significant positive correlation between lnc RNA SRA and BMI(r = 0.631, P = 0.008).6. The individuals with obesity exhibited significantly higher lnc RNA SRA expression than that with lean in PCOS after adjusting for age.Conclusion 1. Our current study presented the first evidence that the lnc RNAs CTBP1-AS and SRA are abnormally up-regulated, and associated with PCOS in Chinese population.2. Abnormal CTBP1-AS expression is a risk factor for PCOS. CTBP1-AS might contribute, at least to some extent, to the etiology of PCOS by modulating the AR activity.3. Elevated lnc RNA SRA might be an important mediator in adiposity-related processes in PCOS for susceptible individuals. |
