Effects Of Bone Morphogenetic Protein - 7 (BMP - 7) On The Expression Of NF - κB P65 And Bax In Cerebral Ischemia Reperfusion Injury

Objective: To study the neuroprotective effects of bone morphogenetic protein-7(BMP-7) in rats models of cerebral ischemia-reperfusion injury, whether it is related with inhibitors of apoptosis proteins NF-κB and Bax.Methods: A total of 70 adult healthy male Wistar rats were divided into three equal groups(except 10 failure model): the sham-operating group, model group, BMP-7 treated group. They were established as middle cerebral ischemia-reperfusion(I/R) injury model by thread tying method. The rats treated with BMP-7 were immediately intervened with BMP-7(0.1 mg·kg-1) via caudal vein after I/R, meanwhile the rats in the other two groups were injected with equal volume of saline. After reperfusion 24 hours, the rats’ neurological deficits scores were assessed; the apoptotic volume of crerbral were detected in TUNEL assay; the expression of apoptosis related proteins NF- κ B and Bax were described by means of immunohistochemical(IHC) method.Results: There were no neurological function imperfection and infarction discovered in the sham-group. Compared with model group after I/R 24 hours, the neurological deficits scores of rats, infarction volume and apoptotic cells in treatment group were decreased obviously. And the differences between the two groups have statistical significance(P<0.05). The results of IHC implied that the MD of proteins NF- κ B in BMP-7 treated group were higher than the model group and that of Bax were significantly lower than the model group. And it has statistical significance(P<0.05).Conclusion: BMP-7 play an important role in I/R injury neurons. It provides protective effect on cerebral ischemia-reperfusion injury through promoting the activity of NF-κB which resists apoptosis and decreasing the expression of Bax which promote apoptosis.