| Purpose:This experiment by means of polymerase chain reaction(PCR) and enzyme-linked immunosorbent assay test(ELISA) methods, "resolving Carbuncle Kui Kang" decoction "detoxification" and "strengthening the spleen and stomach", "Tora saprophytic flesh" three kinds of therapeutic methods for acetic acid in the gastric mucosa in rats with gastric ulcer TFF-2 with respect to effects of expression and content.Material and method:1. Modeling methods: Animal models of acute gastric ulcer induced by acetic acid. 2. Animal groups: laboratory animals were divided into 13 groups, group 10, B-E 12 per group, per group: 10 F-M, female and male half, grouped as follows: A group: normal group, 10. B group: model group, 12. Group C: omeprazole treatment group, 12. Group D: xiaoyongkuidekang treatment group, 12. Group E: Qingrejiedu 12 days of treatment group, 12. F group: the spleen and stomach 12 days of treatment group, 10. Group G: supporting the saprophytic muscle 12 days of treatment group, 10. Group H: Qingrejiedu 8 days of treatment group, 10. I group: the spleen and stomach 8 days of treatment group, 10. Group J: supporting the saprophytic muscle 8 days of treatment group, 10. Group K: Qingrejiedu 4 days of treatment group, 10. L group: the spleen and stomach 4 days of treatment group, 10. Group M: supporting the saprophytic muscle 4 days of treatment group, 10. 3. Experimental methods: adaptability of laboratory rats fed for 2 days in each group, 3rd day fasting for 24 hours free access to water. Separately in each group according to the 2ml/kg standard, with 10% solution of chloral hydrate anesthesia, fastened to theoperating tables, exposed chest and abdomen, lower abdomen disinfected with iodophor on the left rib, cut 3cm incision, exposing muscle and cut muscle layer, use tweezers to pull out the abdominal cavity and flattening of the stomach. At the junction of gastric antrum and body injection of acetic acid 0.02 ml 100% until the gastric serosal layer appeared about rings 3mm white ulcers, also satisfied the stomach in the abdominal cavity, layer-by-layer suture and muscle, skin, and iodophor disinfection. Normal rats implemented sham, how this time, but only the abdominal cavity is opened, after exposure to stomach, not injecting glacial acetic acid, then stitched up. 4. Methods of administration: The rats in group A-G from second days after irrigation model replication of Medicine(co infusion medicine12 days), The rats in group H-J from sixth days after irrigation model replication of Medicine(co infusion medicine 8 days), The rats in group K-M from tenth days after irrigation model replication of Medicine(co infusion medicine 4 days), Give the traditional Chinese medicine decoction or controlled drugs by gavage for 1 times at 10 everymorning, were by gavage for 14 days. 13 days before the gastric lavage of rats during the normal diet,drinking water, fourteenth days of fasting, but the water. Intragastric administration of drugs specifically taking standards are as follows: Group A: normal control group, body weight daily in accordance with 1.25ml/100 g standard with physiological saline; B group: model group, according to the 1.25ml/100 g standard of body weight daily with saline; C group: the control group used omeprazole omeprazole, aqueous solution according to 1.04ml/100 gstandard body weight by gavage; D group: the traditional Chinese medicine treatment group, according to the 1.25ml/100 g standard of body weight daily "xiaoyongkuidekang" decoction; E, H, K groups: heat detoxification treatment groups according to bodyweight, daily 1.25ml/100 g standardwith a group of decoction; F, I, L group: the spleen and stomach treatment groups according to body weight, daily 1.25ml/100 gstandard with two groups of decoction; G, J, M group: supporting the saprophytic muscle groups treatment group, according to the 1.25ml/100 gstandard of body weight daily with three groups of decoction. Weekly review the weight, timely adjust the dosage. The above four groups of rats were executed in 2-4 hours after the last administration, and materials. 5. Observe the Carbuncle Kui Kang "decoction" detoxification "and" strengthening the spleen and stomach "," Tora saprophytic flesh "three kinds of therapeutic methods for acetic acid in the gastric mucosa in rats with gastric ulcer TFF-2 with respect to effects of expression and content.Results:1.Experimental results show that used alone, "resolving Carbuncle Kui Kang" decoction of detoxification, invigorating the spleen and stomach, occasional saprophytic muscle drugs can enhance the acetic acid in gastric mucosa of rats with gastric ulcer TFF-2 content and expression, promote ulcer healing.2.Treatment time under the same conditions, "resolving Carbuncle Kui Kang" decoction of detoxification, invigorating the spleen and the stomach, occasional saprophytic muscle drugs on gastric mucosa of rats and expression levels of TFF-2 in no significant difference, but the effect is weaker than "resolving Carbuncle Kui Kang" decoction. 3.The results show that with the extension of time, heat-clearing and detoxifying, strengthening the spleen and stomach, occasional saprophytic muscle drugs on gastric mucosa of rats and expression levels of TFF-2 in gradually raising the numerical increase was positively correlated with time. 4.Experimental results show that strengthening the spleen and stomach and saprophytic muscle support for 12 days, 8 days group TFF-2 in the relative expression levels and no significant differences in content, but treated by jianpi hewei 8 days group TFF-2 the relative expression level and content is greater than the spleen and stomach and saprophytic musclesupport for 8 days in the treatment group, which in the medium term use of spleen and stomach drug effect is obvious.Conclusion:1.Experiments show that in the pathogenesis of ulcer poisoning fever is the persistence of good and evil, on the medium-term to poison of illness injury. 2.Pathogenic factors of peptic ulcer drug fever virus heat accumulation, heat injury, deficiency of HIV HIV Pathogenesis love evolution.
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