| Purpose：Ulcerative colitis (UC) is a kind of unknown etiology of chronicinflammatory diseases of the rectum and colon, also known as chronic nonspecificulcerative colitis. The clinical features of chronic inflammation in colonicmucosa of ulcer with epithelial layer, severity ranging from recurrent attacks,mainly manifested as diarrhea, bloody stool, mucous stool, fever, anemia, canbe complicated by toxic dilatation of intestine, intestinal bleeding, intestinalperforation. Modern research indicates that cytokines play an important rolein ulcerative colitis, inflammatory response and immune response. InterleukinProinflammatory Cytokines was known to be mediated by cytokines in thepathogenesis of UC, plays an important role in the pathogenesis of ulcerativecolitis. Transforming growth factor is maintain a kind of important medium ofmucosal integrity. This experiment for the purpose of the establishment ofulcerative colitis model in rats, changes of traditional Chinese medicineQingchang decoction in general physiological state before and after thetreatment of ulcerative colitis in rats, and changes of inflammatory cytokinesIL-1and transforming growth factor β TGF-β in serum and intestinal mucosa,so as to investigate the mechanism of the decoction of Chinese medicine QingchangDecoction in the treatment of ulcerative colitis.Material and method：1.AnimalSpecific pathogen free spraugue dawley rats, Rat aged11to12weeks, Eachbody weight198-202g,A total of40(Liaoning Changsheng Biotechnology. Co,Ltd)2.Methods(1)Animal grouping and treatment40male SD rats were randomly divided into five groups: normal control group, model control group, Qingchang decoction treatment group, the Xiang lian pilltreatment group, the mesalazine treatment group. According to animal and humanbody weight dose conversion coefficient table conversion, conversionof Qingchang group that oral administration of0.72g/ml, Xianglian group was0.05g/ml, the mesalazine group was0.02g/ml. The same volume of saline modelgroup rats daily gavage, the blank group were fed normally.Results：1. Normal group of15rats in the experimental process within13days of flexibleresponse, appetite, no diarrhea stool, the weight increased gradually. The modelgroup and three treatment group groups in the stomach first days of diseaseactivity index (DAI), model group, Mesalazine group, Xianglian group, clearingthe DAI score in group and the normal group were significantly different (P<0.01),no significant difference between the model group and three treatment groups(P>0.05), there is no significant differences between treatment groups (P>0.05),The fourth day, DAI score and the model group and three treatment groups hadsignificant difference (P<0.05), For eighth days, DAI score decreasedsignificantly compared with the model group, with statistical significance(P<0.05). For Twelfth days, DAI score decreased significantly compared with themodel group, with statistical significance (P<0.05), but there is no obviousdifference between them (P>0.05).2. Tissue damage observed: Eye tissue damage: blank group: colonic mucosalfinishing, texture clear, no inflammatory exudation and edema. The model grouprats colon mucositis of patients, colorectal wall can be observed obviously edemaand congestion, with the surrounding tissue adhesion. The colonic mucosalsurface is uneven, appeared different degree of erosion, ulcer, thickening andedema, mainly in the distal colorectal lesions. Mesalazine group: colonicinflammation compared with model group reduced, slight inflammatory exudation,edema, hyperplasia part. Xiang lian group: part of the colon wall thickeningof colon mucosal inflammation in rats, compared with model group is light, no significant edema, inflammatory exudate. Qingchang group: colon inflammationcompared with model group, the colon mucosal surface without hemorrhage and edema,no ulcer, slight inflammatory exudation. Under the light microscope: the modelgroup obvious inflammatory reaction disappeared, different degreesof glandular structure, intestinal mucosa epithelial cells showed that alarge area of ulcer inflammatory cells. Mesalazine group arranged glandsstill irregular, with some small area of ulcer, infiltration of inflammatorycells. Xiang lian group intact muscular layer, cells arrangedin irregular, scattered in the ulcer. This group of treatment of coloniclesions were improved, each layer is complete, the gland in a regulararrangement, a small area of ulcer.3. Expression of serum IL-1β, β IL-1β TGF-: rats in the model group increasedsignificantly, TGF-β decreased significantly, the difference wasstatistically significant (P<0.05), Each treatment group comparisons on IL-1β effect: Qingchang group, Mesalazine group and model group were significantlydifferent (P<0.05), the differences between the two groups was no significant(P>0.05), Xianglian group and model group had no significant difference (P>0.05),Each treatment group comparisons on TGF-β: the treatment group and model groupwere significantly different (P<0.05), Mesalazine group and Xianglian group,this was no significant difference between them (P<0.05).4. Expression of intestinal tissue in each group, IL-1β TGF-β: model groupcompared with normal group: IL-1β intestinal tissue in model group ratsincreased significantly, TGF-β decreased significantly, the difference wasstatistically significant (P<0.05), Each treatment group comparisons on IL-1β: the treatment group and model group were significantly different (P<0.05),Mesalazine group and Xianglian group, Qingchang difference between groups wasnot significantly (P>0.05), compared the difference of Qingchang group andXianglian group (P<0.05) Each treatment group comparisons on TGF-β: thetreatment group and model group were significantly different (P<0.05).Nosignificantly difference between the effect of Qingchang Xianglian group and inter group (P>0.05).Conclusion：TNBS in rat model of ulcerative colitis in proinflammatorycytokine IL-1,transforming growth factor β TGF-β expression changedsignificantly, suggesting that the rat model of ulcerative colitis Qingchangdecoction can improve TNBS induced, possibly through down-regulation of IL-1βlevel, up regulation of TGF-β level and work. Reduce inflammation of theintestines, improve immune function, so as to achieve the effect of treatment andprevent. |
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