Genetic Diversity Of Gardenia Jasminoides Ellis By ISSR Analysis

Chinese medicine Gardenia is the plant of Rubiaceae,which namedgardenia Gardenia jasm inoides Ellis dried ripe fruit. this drug begandocumented in"theShenNongencyclopediaofchinesemedicinalherbs ".it iswidely application and its Resistance possessed cold, bitter and to heart,lung, triple burner. with Xiehuo Chufan, jaundice, dark urine, cooling blooddetoxification, heat, swelling topical analgesic efficacy for hot spit bloodnosebleed, discredited astringent pain, fever, irritability, red eyes and throat,canker sores and other evidence of fire. it was mainly produced in Jiangxi,Hubei, Sichuan, Chongqing, Zhejiang, Fujian, Hunan and other provinces.bazhong is the main producing areas in Sichuan gardenia, and Jiangjin also isthe mainstream areas of Chongqing gardenia. They are all authentictraditional Chinese medicine, which has a detailed description in the "ColorAtlas of Chinese herbal medicine". According to modern pharmacologicalstudies confirmed that gardenia with gallbladder， liver， antipyretic,anti-pathogenic,microorganisms,analgesic,anti-inflammatory, sedative effects.ISSR markers (inter-simple sequence repeats) is a labeling techniquesuch as proposed by the Zietkiewicz, using17to22Nucleotides to repeatanchor primer repeat sequences of the amplified fragment[6]. ISSR markersused Primers longer than RAPD, to overcome the RAPD markers fatal flaw ofpoor stability and reproducibility,and its operation is very simple, reproducible, polymorphic more abundant.DNA molecular markers in recent years developed a new high-techmolecular biology techniques, It is within the spirit of the nucleotide sequencedifferences between different genetic material of an individual organismbased genetic markers, Because it can be a direct reflection of the nature oforganisms from the DNA genetic level, with previous biochemical,morphological and cytological markers has obvious advantages. Thus, moreand more widely used in traditional Chinese medicine researchOur group are keep on studying bazhong gardenia，and confirmed theexact value of its excellent quality and exploitation, also considering that inrecent years, studies on the genetic diversity of gardenia ISSR not beenreported in bazhong, Jiang Jin and Naxi production of gardenia, So this paperto study genetic relationship of these three different origin gardenia，and focuson the middle bar to explore the genetic diversity of germplasm Gardenia, inorder to further improve the bazhong Gardenia scientific, standardized,comprehensive quality evaluation system and the quality of research.