| ObjectiveTo study the protective effects of Yulangsan saponins (YLSS) on acute chemical liver injuries by CC14, AP and D-GalN in mice, research and the may mechanisms.Methods①CCl4, AP and D-GalN were used to induce three acute chemical liver injuries models:The Kunming mice were divided into normal control group (NC), model group (MC), high-, medium-and low-doses of YLSS groups, and positive control group (biphenyldicarboxylate group, BPDC) in each acute chemical liver injury model. Then the mice of NC and MC groups were treated with NS, and all doses of YLSS groups and the positive control group were treated with YLSS (112,56,28mg·kg-1) and BPDC (150mg·kg-1) respectively. All mice were treated with drugs or NS for7consecutive days i.g., qd. One hour after the last administration, each group of mice was induced by CC14(0.1%), AP (300mg·kg-1) or D-GalN (800mg·kg-1) separately, ip, except NC group.②24hours later, The indexes of thymus, spleen and liver in various chemical liver injure mice were calculated; the activities of AST, ALT and ALP, the content of Alb, the ability of T-AOC in serum, and the content of MDA, and the activity of SOD in liver tissue were investigated by spectrophotometry, and the degree of hepatic injury was examined.Results①In CCl4induced mice acute chemical liver injury:The activities of ALT was decreased, the content of Alb and the ability of T-AOC in serum were increased by treatment with low doses of YLSS the indexes of thymus, spleen were reduced, the activities of AST, ALT and ALP in serum were decreased, the content of Alb and the ability of T-AOC in serum were improved, and the activity of SOD was enhanced and the content of MDA in liver were decreased obviously by treatment with medium dose of YLSS. The indexes of thymus, spleen and liver were decreased, the activities of AST, ALT and ALP in serum were reduced, the content of Alb and the activity of T-AOC in serum were promoted, and the activity of SOD in liver were enhanced, but the content of MDA in liver were decreased obviously by treatment with high dose of YLSS. Meanwhile, medium-and high-doses of YLSS could extenuate the de-gree of hepatic injury.②In AP induced mice acute chemical liver injury:The indexes of liver and the activities of AST, ALT and ALP in serum were were decreased by treatment with low doses of YLSS the indexes of spleen were reduced, the activities of AST, ALT and ALP in serum were decreased, the content of Alb and the ability of T-AOC in serum were improved, and the content of MDA in liver were decreased obviously by treatment with intermediate dose of YLSS. The indexes of spleen and liver were reduced, the activities of AST, ALT and ALP in serum were reduced, the content of Alb and the activity of T-AOC in serum were promoted, and the activity of SOD in liver were enhanced, but the content of MDA in liver were decreased obviously by treatment with high dose of YLSS. Meanwhile, medium-and high-doses of YLSS could extenuate the degree of hepatic injury.③In D-GalN induced mice acute chemical liver injury:the activity of ALT was reduced, the content of Alb and the ability of T-AOC in serum were increased, but the content of MDA in liver was decreased by treatment with low doses of YLSS. The index of thymus was reduced, the activities of AST and ALT in serum were decreased, the content of Alb and the ability of T-AOC in serum were improved, and the content of MDA in liver were decreased obviously by treatment with medium dose of YLSS. The index of thymus was reduced, the activities of AST and ALT in serum were decreased, the content of Alb and the ability of T-AOC in serum were improved, the activity of SOD in liver was enhanced, and the content of MDA in liver was decreased obviously by treatment with high dose of YLSS. Meanwhile, all doses of YLSS could extenuate the degree of hepatic injury.ConclusionsYLSS has protective effect on acute chemical liver injury. The mechanisms may be related to attenuating free radical and inhibiting the effect on lipid peroxidation. ObjectiveTo investigate the effects of YLSS againsting CCl4-induced liver fibrosis in rats and discuss its mechanism.MethodsThe SD rats were divided into hepatic fibrosis group and normal control group randomly. The rats of hepatic fibrosis group were induced by intragastric administration (i.g.) of50%CCl4, and the normal control group was given normal saline (NS). The rats of hepatic fibrosis group confirmed by the pathological inspection were divided into2subgroups randomly:Drug intervention group and the model group which were treated with NS. The drug intervention group divided randomly into4subgroups:3different doses (20mg.kg-1,40mg·kg-1and80mg.kg-1) YLSS groups and a positive control group (colchicine tablets0.20mg·kg-1). All rats were treated with drugs or NS for4consecutive weeks by ig.①The behavior, diet, mental state and survival quantity of rats were observed and recorded.②24hs after the last administration of drugs, all rats were sacrificed, and the blood, liver, spleen and thymus were taken quickly. The activities of ALT, AST in the serum and the expressions of SOD, MDA, GSH, GSH-Px in the hepatic tissue were analyzed, the indexes of liver, spleen and thymus were counted, and the degree of hepatic injury was examined.③The relative quantification of Col-I, TIMP-1and TGF-β1mRNA expression in hepatic tissues were detected by RT-PCR.Results ①After the treatment in a month, the survival probability of rats in each dose YLSS group and positive medicine group was higher than the model group.②Compared with the model control group, in all dose of YLSS groups and positive control group, the activities of AST and ALT in serum were decreased significantly (P<0.01), as well as the levels of SOD and GSH in liver was increased significantly (P<0.01), but MDA was increased significantly in liver (P<0.05or P<0.01). Meanwhile, GSH-Px activity was increased in all dose of YLSS groups (P<0.01). And the degree of hepatic injury in high dose and middle dose YLSS groups and positive control group could be lessened (P<0.05or P<0.01); The indexes of thymus and liver in rats were decline by treated with high does YLSS (P<0.01),and index of thymus was reduced by treated with Colchicine (P<0.01).③The relative quantification of Col-I, TIMP-1and TGF-β1mRNA ex-pression in hepatic tissues were detected by RT-PCR.ConclusionsYLSS contribute to the inhibition of CC14-induced liver fibrosis in rats.Its mechanism may relate to effects of in attenuating free radical, inhibiting lipid peroxidation, and restraining expression of related genes. ObjectiveTo observe the effects of Yulangsan saponins (YLSS) and Yulangsan saponins-containing serum (medicated serum) on the proliferation of HSC-T6.MethodHSC-T6were cultured in vitro, and MTT assay was used to evaluate the inhi-bitive effect of YLSS.ResultYLSS could obviously inhibit the proliferation of HSC-T6(P<0.05).ConclusionOne of the inhibiting effects on liver fibrosis of YLSS may be restraining the proliferation of HSC-T6.
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