| In this study, the saponin was obtained from the suspension-cells cultured in the established cell suspension culture system which was induced from the leaflets of Pseudostellaria heterophyll (Miq.). On the study of influence of different combinations of hormone,nitrogen source,phosphorus source,carbon cource,inostol and the cell inculated amount on the Pseudostellaria Heterophyll'cell growth and synthesization of the saponin, the dynamic curves were established in order to the highest saponin yield though cell suspension of Pseudostellaria heterophyll (Miq.).1. Plenty of more uniformly and quickly growing suspension cells were harvested from the MS/NB liquid medium with 0.2mg·L-1 BA, 1.0mg·L-1 2,4-D and 1.0mg·L-1 KT.2. The HPLC chromatograms of saponin extracted from suspension cell were consistent with those from conventional cultivation or tissue culture seedlings. At about 30 days, the total content of saponin was 2.13-2.92mg·g-1(DW) cells, slightly lower than those from the roots of conventional cultivation seedlings, and from the roots of tissue culture plantlets.3. After culture condition optimized for cell suspension culture, the results showed that the best condition was the modified NB medium with 5.0 mmol·L-1 KH2PO4+0.5 mmol·L-1 (NH4)2SO4+2.06mmol·L-1 CaCl2+200 mg·L-1 Inositol +40 g·L-1 sugar and 1.0 mg·L-12,4-D +3.0 mg·L-1NAA+ 0.5 mg·L-1 KT ,and added 20 mL liquid medium with 0.03 g(DW)cell·L-14. Under the optimal culture condition, the dynamic curves between the Pseudostellaria Heterophyll's cell growth and synthesization of the saponin indicated that the Pseudostellaria Heterophyll's cell growth reached to the top at the 24th day and the biomass was 0.3578 g(DW)·20mL-1, and from then the cell's growth quantum was reducing, but the saponin content increased rapidly which reached to the top of 329.7147 mg·20mL-1 in the 45th day.
|
PDF Full Text Request