Cloning Of Pollen Specific Protein Gene In Chimonanthus Praecox And Construction Of Expression Vector

The Chimonanthus praecox. is the fallen leaf bush, originally it produces in places such as Jiangsu, Zhejiang, Anhui, Hubei, Jiangxi and Shanxi.It has been cultivated for a long time in our country.As a famous good winter flowers, the Chimonanthus praecox possesses beautiful colour and pleasant fragrance, and besides ,The flowering season of the Chimonanthus praecox could last for three months long. The flower may withdraw the essential oil, and be a medicine for the disintoxicating and cold.The flower bud oil can cur scalds, the root and the stem can make the town to cough, to stop breathes heavily.However takes one kind of precious plants,not actually have the reseach for the chimonanthus praecox in molecular biology.Under the premise of the advance in the molecular biology technology, people has discovered that in the anther growth and the pollen mature process, has the massive genes expressed. Many of them has been in the anther and the pollen organization specificity, And along with time passing, the disquisition of the anther and the pollen specificity expression gene and its the regulative sequence already becomes the research the hot spot.These are studied on that—Separated the pollen specific protein gene from the chimonanthus praecox cDNA library which has been constructed by our laboratory.And has carried on the molecular characteristic analysis to it as well as to carry on the function to its possible function to forecast, And constructed this pollen specific the protein gene to express the carrier.Its mainly research as follows.1.Get Cppspl gene From cDNA librara which has constructed completes stochastic choice library clone, then distill plasmid DNA , and then, use ABI3700 DNA sequence the DNA —...