9 self-bred lines of Chinese chive were used to study the relationship between the changes of physiological index and the low temperature tolerance of leek under 5/0(day/night) and 0/0(day/night)℃stress and to find out the most suitable method of evaluating the low temperature tolerance ability. Then by using 6 self-bred lines of Chinese chive, 15 combinations were made according to complete diallel design Griffing(II) to identify the low temperature tolerance ability of the parent and the combinations. Moreover genetic laws were studied. The main results were as follows:1. Under low temperature stress, the contents of MDA and soluble sugar and conductivity leakage increased, the CAT activity and chlorophyll content decreased. At the early stage of treatment, the activities of SOD and POD and the content of soluble proteins and proline increased, with the stress time went by, and then decreased.2. Before the stress, there was slight difference in the indexes of the varieties. Stressed for 7 days under 5/0(day/night)℃, there were notable difference in the contents of MDA, soluble proteins and proline, the activities of SOD and POD and conductivity leakage. Stressed for 5 days under 0/0(day/night)℃, there were notable difference in the contents of MDA, soluble proteins and proline, and the activities of SOD and POD. These indexes had positive correlation with the low temperature damage, so can be used as identifying index of the low temperature tolerance ability.3. The correlation analysis and path analysis about the indexes showed that: stressed for 7 days under 5/0(day/night)℃, the contents of MDA, conductivity leakage, soluble proteins and praline were positively correlated to each other. The correlation coefficient of the function data of the indexes with low temperature damage was -0.946. So the function data of the indexes can be used to comprehensive evaluate the ability of low temperature tolerance. Stressed for 5 days under 0/0(day/night)℃, the correlation coefficient was -0.906, can be used to comprehensive evaluate the ability of low temperature... |