Study On Chemical Compositions In The Fruiting Body Of Hericium Caput-medusae

Hericium spp. are valuable edible and medical fungi. The medicines made from the concentrated ferment liquid of Hericium caput-medusae(Bu\l.:Fr.) Pers show obvious effects on gastricism, gastic ulcer and other digestive system diseases in clinical applications. In addition, many researches in medicine and pharmacology have proved that the fungi in Hericium spp. have certain therapeutical effects on improving immunity, restraining cancer, reducing blood pressure and blood sugar, and so on. Therefore, in recent years studies on chemical compositions in Hericium spp. are gaining more and more recognition.Studies on chemical compositions of Hericium spp. were mainly restricted in polysaccharide, fat acids and their derivatives, as well as some nutritional compositions such as proteins, amino acids and trace elements both in China and aboard, while studies on organic small molecular compositions were fairly scarce. H. caput-medusae is a main species in genus Hericium, whose compositions were studied much less than other species in this genus. It is significant to make its chemical compositions clear and to make further studies on its medical effects.Solvent extracting method was employed to thoroughly extract chemical compositions from the fruiting body of H.caput-medusae. Powder the dried fruiting body of H. caput-medusae, soak and extract the powder with 95% ethanol at room temperature for many times. The ethanol extract was combined and distilled at 40℃ at a reduced pressure to obtain ethanol concentrated extract liquid. Laid at room temperature, the ethanol concentrated extract liquid divided into four obvious layers. After filtration, the extraction was gained by continuing concentration. The preliminary tests revealed that the test phenomena of sterols were comparatively obvious and positive.Systematic solvent separation, two phases solvent extraction, chromatography, crystallization and re-crystallization methods and diverse separation technology routes were applied to separate and purify the ethanol extraction of the fruiting body of...