Study On Rapid Propagation And Rooting Ex Vitro Of Gardenia Jasminoides Eills

This study was carried out to investigate the effect of proliferation, rooting, and acclimatization of Gardenia jasminoides Eills for large-scale culture. In order to reduce cost of plantlets in vitro, the feasibility of bioreactor culture during proliferation stage and several factors affecting ex vitro rooting were also investigated.Plant growth regulators effected proliferation and rooting of Gardenia jasminoides, the result showed: MS medium with BA of 1.0mg/L and NAA of 0.1mg/L was favorable for differentiation and growth of shoots. NAA was more effective than IBA and IAA for rooting, optimal plantlet growth and 100% of rooting were found in 0.1 mg/L NAA treatment. In addition, standard MS strength (1.0MS), 60mM or 90mM nitrogen, 40 mM NO₃^- mixed 20 mM NH₄⁺ treatments, and 30g/L sucrose were suitable for proliferation of Gardenia jasminoides in vitro. Proliferation and shoot growth were also promoted by ventilation of vessels, a higher proliferation rate and favorable growth were obtained by treatment of 0.5 cm ventilation diameter, however succulent growth of shoot was found in non-ventilation treatment (0 cm). Otherwise less adventitious shoots were obtained in large ventilation treatments (over 0.5 cm).To investigate the feasibility of bioreactor culture during proliferation stage, three culture methods, namely immersion, raft and ebb & flood culture were compared. Immersion culture resulted in highest adventitious shoots numbers (7.1) among the three methods, but showed a large of curled leaves. In ebb & flood culture , although the proliferation rate was lower than immersion culture, but fewer curled leaves was. observed. Raft culture was not suitable for proliferation of Gardenia jasminoides.The feasibility of rooting ex vitro was found during rooting stage. Root formation and growth were batter in foam than perlite treatment; pH and EC in...