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Studies On Bioactivity Of Extracts From D.stromonium Against Several Harmful Creature Of Herbal Medicines

Posted on:2007-09-02Degree:MasterType:Thesis
Country:ChinaCandidate:W YangFull Text:PDF
GTID:2133360185473852Subject:Pesticides
Abstract/Summary:PDF Full Text Request
The relation between the secondary material and the competition for existence and chemistry defense mechanism of the plant have been realized. People have carried out the research and exploit of botanical pesticides. In this paper, the active ingrdients were extracted from the leaves and seeds of Datura stromonium L. Biological activity to Rhizoctonia DC, Brevicoryne brassicae L., Pieris rapae Land Tetranychus cinnabarinus B was studied. The results were as follows:1 Six kinds of extracts were extracted systematacially from the leaves and seeds of D. stromonium with petroleum ether, chloroform and ethyl alcohol, respectively. Among the extracts, the extraction rates of DS21 and DS22 werel4.34% and 13.33%, respectively, higher than other extracts,.2 The results of premilinary study of six extracts' bioactivity indicate that(1) The inhibition rates of DS13 and DS23 of 2 mg/ml against Rhizoctonia DC, were 36.50% and 35.07%, respectively, after extract treatment of 4 days(2) DS11, DS21, DS12 and DS22 to B. brassicae had better bioactivity. After extract treatment of 24h, the emendation death rate of DS11, DS21, DS12 and DS22 of 20 mg/ml to Brevicoryne brassicae was 100%, 91.49%, 100% and 86.40%, respectively. (3) DS11-1, DS11-2, DS11-3, DS12-1, DS12-2, DS12-3 to P. rapae had no evident bioactivity, after extract treatment of 24 h.(4)DS13 and DS23 to T. cinnabarinus had better bioactivity. After extract treatment of 24 h, the emendation death rate of DS13 and DS23 of 20 mg/ml was 73.87% and95.48%, respectively.3 Inhibittion effect of DS13 to to Rhizoctonia DC was further studied. The results suggest that after extract treatment of 16 days, the emendation death rate of DS13 of 10 mg/ml was 73.85%.4 The touch-kill, anti-feed and endosmose activity of DS11, DS12, DS21 and DS22 to B. brassicae were systematacially studied. The results suggest that DS11, DS12, DS21 and DS22 to B. brassicae had good touch-kill activity. After treatment of 6h, the...
Keywords/Search Tags:D.stromonium, Herbal medicine, B. brassicae, T. cinnabarinus, Rhizoctonia DC, Bioactivity
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