Toward Mapping Of QTL For Waterlogging And Pre-Harvest Sprouting Resistance In Wheat

Abiotic tolerance are agronomically important but biologically complexed traits, which make it very difficult to breed for stress or tolerance. To identify genes for waterlogging and pre-harvest sprouting tolerance in wheat, QTL mapping study are conducted using two recombinant inbred line population.To identify QTL conferring waterlogging tolerance, recombinant inbred lines (RIL), developed from the cross of Triticum macha (accession #49) (tolerant to waterlogging) and Ningmai 3 (susceptible to waterlogging) and the parents were evaluated for waterlogging tolerance in pots and water-conserving pools, respectively, by saturating the soil bed with water for about 20 days at the booting stage. The ratio of the number of green leaves of the treated plants to that of the untreated plants of each line (green leaf relative index, GLRI) was calculated to represent the waterlogging tolerance. The GLRI showed a continuous normal distribution in the RIL population. Twenty of 164 RAPD markers and 332 of 835 SSR markers screened detected were polymorphism between the two parents, Through ANOVA, four chromosome regions were found to be significantly associated with GLRI. One region on chromosome 5A were significantly related to GLRI in both trials, explaining 10.1% and 11.6% of the phenotypic variance, respectively. The alleles of the QTL for higher numbers of greens leaves were all contributed by Triticum macha accession #49. These results are useful to explore genes underlying the waterlogging tolerance.A population of 117 RILs was developed from the cross of Fengchan 3 (resistant to...