The Hybrids Of Intergeneric Hybridization Between Brassica Species And Raphanus Sativus And Their Identification By SSR

Hybridization is made between Brassica species and Raphanus sativus which is an important way for setting up germ plasm resource storerooms of Cruiferae, but improving the ratio of setting seeds is indispensable to using intergeneric hybrids. By spreading on stigmas, Gly(100mg/L), GA₃(60mg/L), NaCl(5%) solution could accelerate fertilization and improve development of ovaries and ovules in this study. By ovary culture, some hybrids were obtained from the hybridization between Brassica and Rsativus. Moreover, a way of plant tissue culture was erected to improve culture efficiency on intergeneric hybridization between Brassica and R.sativus. These hybrids were further confirmed by the analysis of SSR marker. The main results were as following:1. It was found that crosses between Brassica and Rsativus species were highly incompatible. Total 2 240 stigmas were pollinated, and 128 pods and 196 pollinating seeds were harvested. After these seeds were further sowed, F₁ plants showed the same morphological characters as their female parents, suggesting that F₁ plants were matrolinous hybrids.2. Parents genotypes and crossing modes were the main factors on affecting ratio of setting seeds in the crosses between Brassica and Ksativus. Different compatibilities were found among various combinations, and the compatibility of B.napus ×R.sativus was higher than both that of B.juncea ×R.sativus and B.campestris× Rsativus, The compatibility of B.campestris×R.sativus was the lowest among three combinations. In reciprocal crosses , the compatibility of Ksativus ×B.campestris was the highest among R. sativus ×B.napus , Ksativus ×B.juncea and Ksativus ×B.campestris. The compatibility of combimations were affected by crossing modes, the compatibility with the Brassica as the female parent was higher than that of the reciprocal crosses.3. In the cross of Brassica and Ksativus, Gly(100mg/L) and GA3(60mg/L) solution could improve development of ovaries and ovules by spreading stigmas. The effect of these hormones were different among the above combinations, indicating that the effect of hormones was relevant to parents genotypes.