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A Genetic Map Of Blumeria Graminis Based On AFLP Markers And Avirulence Genes

Posted on:2007-06-13Degree:MasterType:Thesis
Country:ChinaCandidate:L H MeiFull Text:PDF
GTID:2133360185455367Subject:Plant pathology
Abstract/Summary:PDF Full Text Request
The inheretance of avirulence towards wheat mildew resistance was analysed in a cross between Blumeria graminis f.sp. tritici isolates CPW-1 and E17. The result shows that the avirulence matching resistance genes Pm3b and Pm3f is controlled by one gene. The avirulence matching Pml3 is controlled by two avirulence genes. Each of avirulence matching Pm2, PmXBD, Pm4a, and Pm4b is controlled by three genes. The inheritance of the avirulence matching Pm2 and PmXBD is first described.A total of 208 random primer pairs were used to find polymorphic DNA fragments. Result shows that 105 pairs of primers can amplify polymorphic DNA fragments between the parent isolates. 22 pairs of primers that selected from all random primers were used to make AFLP analysis between parents and 88 progeny. Result shows that 17 loci linked in 5 groups. The total diatance is 125 cM with the averaged distance of 7.35 cM. The remaining 34 loci were not found to be linked with each other. The reason may be that the markers obtrained were too scattered so that they can not be linked to each other. A denser and finer map is needed.
Keywords/Search Tags:wheat powdery mildew, avirulence gene, genetic analyse, AFLP, genetic map
PDF Full Text Request
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