AFLP Analysis Of Population Genetic Structure Of Wheat Powdery Mildew In Anhui Province

Wheat powdery mildew,caused by Blumeria graminis f.sp.Tricici,is one of the major diseases in Anhui Province.The Blumeria graminis population genetic structure and the relationship among sub-populations in Anhui were explored by molecular biology methods in this study.The results will be significant for wheat powdery mildew epidemic prediction and effective control in Anhui.From 2009 to 2015,the infected leaves were sampled in 20 counties and cities of wheat powdery mildew often occurred regions such as Anqing,Suzhou,Hefei,Huainan,etc.After single spore isolation and purified,a total of 104 isolates were obtained.According to the geographical distance,the 104 isolates were classified as nine geographical populations,and according to the sampling year the isolates divided into five year populations.Population genetic structure of Blumeria graminis in Anhui was studied by fluorescence AFLP molecular marker system.The conclusions were as follows:(1)In this study,the genomic DNA of Blumeria graminis was extracted by the method of RELI-g Mini Kits,which was adble to meet the AFLP analysis of genomic DNA,which greatly improved the efficiency of the research.(2)Accorded to the geographical location,Anhui division into Anqing Xuancheng(aqxc),Hefei Luan Ma On Shan(hflam),Suzhou,Bozhou szbz,Fuyang Bengbu Huainan(fybbhn)four geographical groups.The results showed that population genetic diversity level in northern Anhui pathogen populations(szbz,fybbhn)was higher than the southern population(aqxc)and central population(hflam).This might be caused by that northern Anhui wheat planting area far more than southern and mid-areas.Certain genetic variation existed among four popoulations,but can not reached a significant level.There are common genotypes among the four populations,but Anqing Xuancheng group(aqxc),Fuyang Bengbu Huainan(fybbhn)group,Hefei Lu’an saddle hill(hflam)population phylogenetic relationships among the three populations was similar,and the genetic difference of northern population szbz and the three groups were relatively far.(3)accordance to the sampling time,the Anhui Blumeria graminis isolates were division into 2009,2010,2011,2014 and 2015 five year populations.The results of the study showed a certain level of genetic diversity among five year populations,but did not reach the significant level.High AFLP genotypes diversity was detected in 5 year populations.Principal component analysis results suggested that the trend of more overlap in more recent year populations.Cluster analysis showed that in addition to the 2010 group,the other four year groups were clustered by the early or late of sampling year.(4)To furtherly analysis of Anhui Tianmu Mountain pathogen population impact on other surrounding areas,this study showed that level of genetic diversity of the population in Tianmu Mountain area higher than other surrounding areas,and the order of the genotype diversity was: Xuancheng(XC)> Anqing(AQ)> Ma’anshan Hefei(HFM)>Lu’an city(LA),and principal component analysis in Tianmu Mountain area of Xuancheng(XC)compared to several other groups,scattered amplitude is more broad,these results suggest that the Anhui Tianmu Mountain area of Blumeria graminis population had bigger impaction on the surrounding to other parts of Anhui.This may be caused by the special geographical conditions in Tianmu Mountain area more suitable for Blumeria graminis oversummering smoothly,so wheat powdery mildew in Anhui Tianmu Mountain area should be paid more attention.