Characterization Of Low-molecular-weight Glutenin Subunit Genes From Wheat Variety Chuanmai 42

Using PCR-amplification method, the coding sequences of two low-molecular-weight glutenin subunit (LMW-GS) were isolated from a new wheat variety Chuanmai 42, designated as LMWCM42-1 and LMWCM42-2. The deduced amino acid sequences of these two genes are highly homologous (81.76%). The main divergences are in the repetitive and C-terminal domains. Alignment analysis revealed a strong similarity between the two sequences and known LMW-GS genes. The main results were as follows:1. Using primer pair P1 and P2, a 850bp band with the expected length of coding region of LMW-GS gene was amplified from genomic DNA of Chuanmai 42.2. Sequencing of positive clones resulted in two different sequences, designated as LMWCM42-1 and LMWCM42-2. They are 846bp and 912bp, and could be translated to 281 and 303 amino acids, respectively. BLAST analysis confirmed the two sequences are LMW-GS genes.3. The alignments showed a high level of sequence identity among the two sequences and known LMW-GS sequences derived wheat and its related species. LMWCM42-1 was more homologous with AY831795, while LMWCM42-2 were more orthologous with AY831792, AY831790, AY831789, AY296753 andAB062872.4. The clustering analysis suggested that the two genes were more orthologous with the genes at the Glu-D3 locus in wheat, and might be encoded by the locus of Glu-D3.