Study On The Special Wheat Matierial R149 Based On Cyto-biology And Molecular Biology

Advanced wheat cultivar R149 whose parents are common wheat (Triticum aestivum) and rye (Secale cereals) is bred by new chromosome engineering ways "The use of monosomic rye addition lines for transferring rye chromatin into wheat" . Using the C-banding technique, genome in situ hybridization, and protein seperation technique and PCR studied R149 cultivar systematically. In order to verify the theory "induction of small-segment-translocation" . The studies substantiated feasibility of the theory.1. The study results suggested that the monosomic added rye chromosomes in wheat could induce translocation between wheat and rye chromosomes effectively and that the added monosomic could induce small segment translocation (SS translocation) with high frequency. The theory "induction of SS translocation" is significant in plant breeding compare with other ways of induction of translocation.2. The results of traditional cytology techniques showed that R149 has a normal cytology behavior and stable genetic system. Whereas, the plants exhibited phenotypic alteration compared with its wheat parent.3. The results of the GISH indicated that the control materials showed alien chromosome clearly and R149 had a relative regular hybridization site along the special chromosomes. Therefore, we could conclude that it is possible that R149 had some segments of the rye chromosome.4. Using the primers of specific repeated sequence from S. cereale amplified the R149, the results showed that pscll9.1, pscH20 could amplified the products correctly.5. Based on SDS-PAGE analysis, R149 showed no rye special glutenin protein.However, it has a terrific quality of making bread wheat.