| In this thesis, HhaI polymorphisms of halothane gene in 94 Tibetan pigs and Pvu II polymorphisms of ESR gene in 88 samples and Apa I , Pvu II , BstXI, Hae II polymorphisms of growth hormone gene in 94 Tibetan pigs were detected by PCR-RFLPs.The results of polymorphisms of restriction enzyme sites were indicated as follow: the genotypes of halothane were NN. This showed that Tibetan pig had the strong capability of resisting stress. The frequency of AA genotype of the ESR gene was 11.36%. AB was 64.77%. BB was 23.86%. The frequency of A allele gene was 43.75% and B was 56.25%. There was a significant difference between A and B allele gene (P<0.05).B allele was a dominating gene of the porcine number born. The high frequency of B allele indicated that Tibetan pig had high potential competency of reproduction.After the growth hormone gene was digested by Apa I, two alleles and three genotypes (AA, AC and CC) were detected. The frequency of AA, AC and CC genotypes were respectively 7.5%, 24.5%, 68%. The frequency of A allele gene was 19.8% and C was 80.2%. There was a significant difference between A and C allele gene (P<0.05). BstXI digestion revealed two alleles and three genotypes (AA, AB and BB). The frequency of AA, AB and BB genotypes were respectively 2.1%, 11.7%,86.2%.The frequency of A allele was 8.0% and B was 92%.The frequency of A and B was a signification difference (P<0.01) . PvuII digestion revealed two alleles and three genotypes (AA, AB and BB). The frequency of AA, AB and BB genotypes were respectively 6.4%, 7.5%,92.5%.The frequency of A allele was 7.5% and B was 92.5%.The frequency of A and B was a signification difference (P<0.01) . Three alleles and three genotypes (AA, BC and BB) were shown after digestion with Hae II. The frequencies of the three genotypes were respectively 2.1%, 22.4%, 75.5%. The frequency of A allele wasl4.5 %, B was 74.5% and C was 11%. There was a very signification difference among three alleles (P<0.01). |
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