A N-Metabolic Studying Of High-protein Maize By Transmitting Exogenous Soybean DNAs Into Maize

This text is a physiology and bio-chemistry studying of high-protein maize by transmitting exogenous soybean DNAs into maize.The results shows:(1) DC grain germination rate, germination strength show the higher than CK grain; the DC stub is average high degree 1.75 m, tassels are high 1.35 m the CK stub is average high degree 1.80m, tassels are high 1.45 m;DC ismore illess than CK .(2) variety regulation that corresponding period Pr hydrolases activity express, DCs activity is higher, and can promote the synthesizing of protein and decline, increase the backlog for growing the protein of grain, and is one of the high foundation terms of deal.(3) variety regulation that corresponding period NR activity express, leaves activity for NR obviously higher than grain, under same alike plant the term, exterior supply nitrate level same alike, DC leaf is obvious higher than CK's, supply the grain more nitrogen, and so and finally cause a protein to contain to measure higher than CK.(4) variety regulation that corresponding period GS activity to express, under the equal educate the term, DC and CK GS for leaf gradually go upping and showing the higher than nitrogen of, store the N for the grain continuously of provide the nitrogen.The ammonia's assimilation is main in the leaf department the mature GS for proceeding, therefore along with grain gradually descend.(5) variety regulation that corresponding period nitrogen and K contain the deal express, and the equal apply fertilizer the term bottom. DC grain the inside nitrogen contain to measure obvious higher than CK, the one of the high reasons.to turn the gene corn the protein contain to measure .(6)DC Pr is higher than CK's.(7).DC root is the vitality show the higher than CK.(8) pass to withdraw the DNA of the soybean bud to use the pollen tube the passage method with the machine its turn into the corn framework from hand over is behind the protein contain to measure to increase, among them that transfer point gene is 22 KD.This point the protein contain the deal DC higher than CK the same of point 250%.