| A endogenic activator protein isolated from Alternaria was a high performance, wide-spectrum, non-toxicity to human beings and livestocks, non-pollutional and novel inducer.The activator protein could activate a series of responses, and activate seed bourgeon and growth and induce immunoreaction to a large number of parasites of plant by binding to the acceptor of cell membrane. In this study, growth status of rice seedling, the dynamic change of some enzymes pertinent to growth of seedling, such as amylase, SDH and PK, the induce resistance to Magnaporthe grisea and the direct inhibition against a large number of plant parasites were investigated after being treated with this protein of different concentration, which concentration was separately 1 μg·mL-1, 2 μg·mL-1 and 6 μg·mL-1. And under the same condition, the difference of gene expression of rice seedlings was also conducted using gene chip. The results were as follows:1.The molecular weight of the activator protein originating from fungi was 42 KD by electrophoresis after being extracted and purified.2.Effect of activator protein on root length and plant height of rice seedlings: When the concentration of activator protein was 2 μg·mL-1, growth of rice seedlings was enhanced. while the concentration was 6μg·mL-1, growth of rice seedlings was inhibited. However, the concentration was 1μg·mL-1, the difference was too small to be observed.3.Effect of activator protein on the activities of enzymes pertinent to growth of rice seedlings:(1). Treated with activator protein, activity of SDH of rice seedlings increased, and was still higher than that of the control after 5 day, respectively. Especially, when the concentration were 2 μg·mL-1, the effect was the most significant, reached 67.1% higher than that of the control, and its lasting time was the longest.(2). Treated with activator protein, activity of amylase of rice seedlings increased.
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