The Effect Of Starvation And Different Fatty Acids On The Fatty Contents And FAS Activity Of Grass Carp

Animals acquired energy from foods everyday, and conversed plentiful energy to fat in liver and muscle. The major fatty acid that for animals store comes from fatty acid de novo fatty acid synthesis, and FAS is key- enzyme, so the concentration and activity of FAS proteins are important to control animals fatty. By using Grass carp as material for 28 days experiments ,the effect of different fatty acids on fatty contents , FAS activity and FAS gene expression were investigated in system. And the effect of starvation on fatty contents and FAS activity of Grass carp were investigated at the same time. The main results are as the following:1. The effect of starvation on fatty contents and FAS activity of Grass carpThe fatty contents and FAS activity in muscles and livers were measured for Grass carp in starvation for 0, 7, 14, 21 and 28 days as treatments, and the Od measurements were made as control. Testing for the difference between the treatment and control resulted in: The fatty contents in muscles and livers decreased all. The reduction of muscles was from 2.45% to 1.43%, and the reduction of livers was from 3.01% to 1.73%. The course of reduction was made up three stages: The first stage (0d-14d), there were smooth descent, 0.01%·d^-1 and 0.03%·d^-1 in muscles and livers;The second stage (14d-21d), there were significantly decreased, 0.11%·d^-1 and 0.10%·d^-1 in muscles and livers; The third stage (21d-28d), there were smooth descent, 0.01%·d^-1 and 0.03%·d^-1 in muscles and livers. The FAS activity in muscles and livers decreased. The reduction of muscles was from 1.31 U/mg to 0.33 U/mg, and the reduction of livers was from 2.18 U/mg to 0.62 U/mg. The course of reduction was made up four stages: The first stage (0d-7d), there was significantly decreased, 0.03 U/mg·d^-1 and 0.05 U/mg·d^-1 in muscles and livers; The second stage (7d-14d), there were smooth descent, 0.02U/mg·d^-1 in muscles and livers; The third stage (14d-21d), there were significantly decreased, 0.05 U/mgd"1 and 0.11 U/mg·d^-1 in muscles and livers; The fourth stage (21d-28d), there were smooth descent, 0.04 U/mg·d^-1 in muscles and livers, but there was significantly decreased to the first and the second stages in muscles.2. The effect of different fatty acids on fatty contents and FAS activity of Grass carpThe fatty contents and FAS activity in muscles and livers were measured for Grass carp feed in 6% different fatty acids, defatted, lard, soybean oil and fish oil for 0, 7, 14, 21 and 28 days as treatments, and the Od measurements were made as control. Testing for the difference between the treatment and control resulted in: The fatty contents in muscles decreased after 28d of different fatty acids: Defatted: 0.13%; Lard: 0.40%;Soybean oil: 0.91%; Fish oil: 1.10%. The FAS activity in muscles was decreased by different fatty acids: Defatted: O.llU/mg; Lard: O.llU/mg; Soybean oil: 0.81U/mg; Fish oil: l.HU/mg. The FAS activity in livers was decreased by different fatty acids: Defatted: O.35U/mg; Lard: 0.63U/mg; Soybean oil: 1.46U/mg; Fish oil: 1.93U/mg. The fish oil and soybean oil have significantly effect on FAS activity (PO.01), but there were no significantly different (P>0.05) between fish oil and soybean oil; The lard and defatted have no significantly effect on FAS activity (P>0.05). 3. The effect of different fatty acids on FAS gene of Grass carpThe fatty contents and FAS activity in muscles and livers were measured for Grass carp feed in 6% different fatty acids, defatted> lard, soybean oil and fish oil for 0, 7, 14, 21 and 28 days as treatments, and the Od measurements were made as control. Testing for the difference between the treatment and control resulted in: The FAS gene expression contents were suppressed by different fatty acids. The FAS gene expression contents were decreased in muscles: Defatted: 8.95%; Lard: 11.05%; Soybean oil: 44.74%; Fish oil: 62.11%. The FAS gene expression contents were decreased in livers: Defatted: 5.71%; Lard: 11.43%; Soybean oil: 42.50%; Fish oil: 66.43%. The fish oil and soybean oil have significantly effect on FAS gene expression contents (PO.01), but there were no significantly different (P>0.05) between fish oil and soybean oil; The lard and defatted have no significantly effect on FAS gene expression contents (P>0.05).