Effect Of Tea Polyphenols And Vitamin E On Broilers Performance And Antioxidant On Under Heat-Stress Conditions

Under the condition of stress and senescent, there will produce too many oxygen free radicals in animal body, which results in abnormal structure and functional of cell membrane, and results in animal disease. Antioxidants can clean out too many free radicals and maintain the health of animals.Tea Polyphenols (TP) is the most available substance in tea.It has strong ability of anti-oxygen. It can eliminate oxygen and grease free radicals effectively. In this experiment TP was applied under the heat stress, combining with VE, which was used popularly in manufacture recently, to study the effect of different TP and VE levels on the performance quality, immunity condition and anti-oxygen ability through feeding test and slaughter test. This can provide reference to the usage of safe efficient antioxidants and substance defending from stress.The experiment was carried out within 4 weeks and 1 weeks beforehand. 162 21-days old AA broilers were randomly arranged to 9 groups with three depiicates of 6 broilers. The trial was designed to double factors of three TP levels(0mg/kg, 20 mg/kg, 30 mg/kg) and three: VE levels(0 mg/kg , 100 mg/kg, 200 mg/kg). The maize-soybean diet was formulated as recommended by Nation Standard (1998).The results of the experiment are as follows:1. Performance: TP and VE had not significant influence on total performance of broilers (p>0.05) . From 1-week to 3-week of heat-stress, TP and VE had significant influence onfeed intake of broilers (P<0.05) ; from 2-week to 3-week of heat-stress, interaction of TP and VE had extreme significant influence (p<0.01) ; during 4-week of heat-stress, TP and VE had significant influence on feed intake of broilers (P>0.05); during 1-week of heat-stress, TP and VE had significant influence on daily weight gain and feed/weight gain of broilers (p<0.05) ; after that there was not significant influence (P>0.05) .2. Immune organ indexes: VE had no distinct effect on the immune organ indexes of broilers (P>0.05) . TP had significantly affected the thymus index of broilers (P<0.05) but no significant influence on spleen and bursa.F indexes was observed (P>0.05). TP and VE had no interaction on these indexes (p>0.05) .3.Index of physiological and biochemical: during 1-week of heat-stress, TP and VE hadnot significant influence on the content of Na, K, total protein and CHOL in the serum(p>0.05 ), there wasn't interaction between them (p>0.05 ); during 4-week of heat-stress, VEhad significant influence on the content of K in the serum (p<0.05) , but TP and VE had not significant influence on other indexes (p>0.05) .4. anti-oxygen indexes: TP had significant influence on serum GSH-px at these two phases(P<0.05), but VE had not(P>0.05). No differences were found on SOD among 5-week broilers fed with diets of different TP and VE (P>0.05), there wasn't interaction between them(p>0.05) . TP had nearly distinct influence on the content of VE in broilers' heart (P<0.05), diets with different Ve levels had great significant influence on it (p<0.01 ), there wasn't interaction between TP and VE (P>0.05) .At the prophase TP and VE influenced the serum T-AOC significantly (P<0.05), the influence of VE was close to great prominence (P<0.05) but no interaction between them was observed. TP and Ve all had no significant influence on serum MDA content at the prophase( P>0.05). but diets with different TP levels influenced the serum MDA content of broilers greatly (P<0.05) .5. meat quality: VE had significantly influenced the content of VE in meat (P<0.05), there wasn't interaction between them (P>0.05) .