Relationship Of Myostatin Gene With Growth Traits By PCR-SSCP In Swine

Lean meat percentage is one of the most important economic traits in swine breeding programs. Myostatin is also one of candidate gene, associated with lean meat percentage in swine. In this study, DNA samples from 250 pigs including three introduced swines and two Chinese native swines breeds. MSTN gene was studied by PCR-SSCP. The effect of different genotype on weight at birth (WB) and weaning weight (WW) was analyzied, in order to provide an approach of MAS for selecting lean meat contant in swine. Two alleles and three genotypes for MSTN locus in the five swine breeds by PCR-SSCP were found .The genotype frequency was significantly different (P<0.01) among breeds. Myostatin gene have two allele gene A,B and three kinds of genotype AA,BB,AB. Two allele genes were found and allele A was the superior gene in five swine breeds.The test of Hardy-Weinberg equilibrium showed that five swine breeds diverged from the genetic equilibrium (P<0.01). A SNP was identified in the 5′-regulator region of the swine myostatin gene. Association of the myostatin genotypes with growth trait are unknown in swine.The present study to analyze the relation of the mutation with the growth trait which including weight at birth (WB) and weight at weaning (WW) by fixed effect model.Five swine breeds were examined. Allele specific breeds for one SNP were confirmed. The effect of breeds on WB and WW was estimated (P<0.01). Excluding interaction between genetypes and breeds, variation of MSTN with weight at birth (WB) and weaning weight (WW) were evaluated by General Linear Model of SPSS 10.0 software package, which showed a significant difference for WB and a non-significant difference for WW.