Studies On The Allelopathy Between Of Glycyrrhiza Uralensis And Caragana Microphylla By Means Of Tissue Culture

Glycyrrhiza uralensis Fisch is an in common use and big a Chinese herbal medicine material in our country, but adopts to accept the process inside will bring the negative influence to the ecosystem environment. Caragana Microphylla Lam livings the defoliation bush for many years, a department prospers, ising a good tree to defends the breeze sand and keeps Water and soil to grow. This text tries to investigate to use together the weather licorice in the district plants compound, then satisfy the society to the need of the licorice, pass again an of caragana the protection function reduces the licorice adopts to accept the breakage that result in to the ecosystem environment. Adopt the method of the tissue culture for the very first time, try hard for to avoid the outsider factor in etc. in soil affect, to licorice with caragana a research for turning feeling function proceeding system, study both possibility that compound plant, Result is as follows:1. Caragana caudex while being partial to the acidity( pH=6.5) term, the MS conduct and actions is basic to culture medium, increase the plant growth regulates 6-BA0.2 mg.L^-1 and IAA0.2 mg.L^-1increases in value the result the best; Increasing the plant growth regulates an IBA0.2 Mg.L^-1 with NAA0.4 The mg.L^-1 takes root the result the best.2. Licorice caudex segment while being partial to the acidity( pH=6.5) term, the MS conduct and actions is basic to culture medium, increase the plant growth regulates a 2,4 D1.2mg.L^-1 with 6- the BAl.0mg.L^-1 increases in value the result the best,1/2 MSs+ the NAA0.5mg/ L^-1 is a superior taking root to culture medium.3.The mixing planting both of Guralensis and C.microphylla result enunci — ation: The stub of the licorice is high, a long and fresh and heavy etc. index sign is all better than its educates alone. C.microphylla growth for exsitting with each other benefit relating to, but passing tissue culture with each other changing and with each other a leafs slice immersing lifting hour of liquid discovering, small piece(1 cm³) tissue culture with low den-sity(1 mg.mL^-1) leaf's slice immersing lifting liquid all to licorice and C.microphylla the function, But the big piece(2 cm³) tissue culture represses the function with the high dens-ity(5-50 mg.mLs^-1) a growth for immersing lifting liquid to licorice and C.microphylla.4.Pass the liquid mutually the chromatogram apparatus analyse, root's secretion andextraction from leafage of caragana contain phenol,Alkene, Fluorene those framework buildup by phenyl. liquorice contain ester. The function mechanism of these compoundses still needs the further research.5.In conclusion, the biology and ecology characteristic of Guralensis is different from C.microphylla.The mixing planting model will not have obviously influence on medicinal. The aim of developing medicinal materials resources and protecting ecological environment will berealized.