Study On Optimum Requirement Vitamin A,D,E Of Lactating Holsteins And Dynamic Change Of Vitanin A,D,E In Alimentary Canal

Part oneIn this experiment ,fifty eight lactating holsteins were chosed and 3 x 3 latin square design were adopted to research the requirement of vitanin A,D,E of lactating holsteins and the effect of different levels of vitanin A,D,E supplemented in the diets on milk fat, lactoprotein, milk suga, SCC,concentration of vitanin A,D,E in milk and serum and so on in two periods,The results showed that:l.The optimum level of dietary supplemental vitanin A,D,E of lactating holsteins is respectively 1250000IU/d, 16000IU/d and 1300mg/d .2.There is no interaction between VA and VE in statistics (P>0.05) ,but it is intensively suggested fom data that there is some interplay between VA and VE.3.There were significantly difference in milk fat, lactoprotein, milk suga, antibodies,SOD,MDA,GSH,NEFA,minerals (P>0.05) .4.As the concentration of vitanin A, E in diets increased,the oncentration of vitanin A,D,E in serum mounted up significantly (P<0.01) .Part twoIn this experiment ,twenty seven lactating holsteins were selected and crossing design were adopted to research the interaction of vitanin A,D,E and the effect of different levels of vitanin A,D,E supplemented in the diets on milk fat, lactoprotein, milk suga, SCC,concentration of vitanin A,D,E in milk and and serum and so on, The results indicated that:1.There is no interaction between VA and VE in statistics (P>0.05) .but it is intensively suggested fom data that there is some interplay between VA and VE.2. There were significantly difference in milk fat, lactoprotein, milk suga, antibodies,SOD,MDA,GSH,NEFA,minerals (P>0.05) .3. As the concentration of vitanin A, E in diets increased,the oncentration of vitanin A,D,E in serum mounted up significantly (P<0.01)Part threeIn this experiment ,three holsteins fitted with permanent rumen fistulate, proximal duodenum fistulate and ileum fistulate were used in 3x3 latin square design and ETDA-Co was used as marker to determine the effect of different levels of vitanin A,D,E supplemented in the diets on concentration of vitanin A,D,E in rumen, duodenum , ileum and serum and the asorption of vitanin A,D,E in small intestine,The results indicated that:1 .As the concentration of vitanin A, E in diets increased,the oncentration of vitanin A,D,E in rumen, duodenum , ileum and serum mounted up significantly (P<0.01) and the deposition of. vitanin A, E in small intestine also went up2. there were significantly difference in concentration of vitanin A, E at different sampling time (P<0.01) .