Overexpression Of Arabidopsis Salt Tolerance-Related Genes Together In Upland Rice And Improvement For Salt Tolerance

Salt stress is one of the environmental factors those greatly limit crop production in the world. Salt stress influences plant growth and development through osmotic stress and ion toxic. Improved salt tolerance could be achieved by Overexpression of a plasma membrane Na~+/H~+ antiporter SOSl or vacuolar membrane Na~+/H~+ antiporter AtNHX1. The previous studies have demonstrated that the activity of SOSl is regulated by SOS2/SOS3 kinase complex, which belongs to the same pathway named SOS pathway, and the tonoplast Na~+/H~+ antiporter is a target of the SOS pathway. So overexpression of Arabidopsis salt tolerance genes together may improve plant salt tolerance more than one gene. This point has been verified in yeast cells.With the utilization of gene-gun technique, the Aribidopsis salt tolerance-related genes were overexpressed or specific expressed in upland rice297. We used the PCR method to verify if the target genes had been transferred into the plants. By now, we have obtained the following results:(1) SOSl gene under a super-promoter and under a specific promoter were transferred into upland rice297, respectively. Using the PCR method, we verified that the target genes had been transferred into upland rice297.(2) AtNHX1 gene drived by a super promoter was transferred into upland rice297. Using the PCR method, we verified that the target gene had been transferred into upland rice297.(3) SOS2 and SOS3 gene under control of 35S promoter were transferred into upland rice297, respectively. Using the PCR method, we verified the target gene had been transferred into upland rice297.(4) The transgenic plants for salt tolerance will be tested in the future.