Effects On Cadmium To Apoptosis Of Spermatogenic Cells And Related Gene Proteins Of Mouse Testicle

Cadmium is known as a harmful metal widely in animals and birds production resided through feedstuff. Cumulation and residue of cadmium could lead animal toxicosis, such as developmental toxicity, reproduction capacity, cancer and even death, etc. The paper indicated effects on cadmium to apoptosis of mouse spermatogenic cells and related gene proteins.By the use of the mouse testicle as animal model, cadmium chloride was given to mice through i.p. and an antagonistic group was designed. The mice were sacrificed in 3d, 6d, 9d after treatment and the testicles were observed the morphological changes of mouse testicles through H.E stain. Apoptotic DNA of spermatogenic cells was extracted from the testicles, then was detected apoptotic DNA ladder formation by agarose gel electrophoresis and apoptosis rate of spermatogenic cells by TUNEL method quantitatively. The immunohistochemistry method was adopted to measure and locate the expression changes of gene proteins -bax and bcl-2 to the induction and inhibition of apoptosis.The results showed that the testicles in the control group and the antagonistic group were normal. The testicles in cadmium poisoning group were damaged more or less. The results of testicles in cadmium poisoning group showed significantly higher than those of the control group and had the correlation with the dosage of Cdch and poisoning time. DNA ladder formation of the poisoning group had been measured by agarose gel electrophoresis. All spermatogenic cells showed apoptosis in different levels. With the increase of the Cdcl₂ dosage and the prolongation of poisoning time, all spermatocytes, spermatids, spermatozoons and spermatogonia showed apoptosis in different levels. The differences among every group were existed significant.Gene proteins-bax and bcl₂ could regulate apoptosis of spermatogenic cells. Bax could develop cell apoptosis and bcl_-2was on the contrary. Both of them could located on the membrane and the cytoplasm.The conclusion showed that Cdcl₂ could lead spermatogenesis dysfunctionbecause of seminiferous epithelium damage in mouse testicle.Sensitivity to cadmium in different spermatogenic cells was different. Spermatocyte was the most sensitive to cadmium, then spermatid, spermatozoon and spermatogonia. Both bax and bcl-2 could control spermatogonic cells apoptosis caused by cadmium. Adding zinc could caused obvious antagonism to cadmium poisoning.