Study On The Localization Of The Sympathetic Neurons Innervating The Liver And Gall Bladder Of The Fowl

The present study revealed the localization of the sympathetic postganglionic neurons and the sensory neurons of spinal nerves innervating the liver and gall bladder of the fowl by the use of retrograde transport of cholera toxin-horseradish peroxidase (CT-HRP) .The CT-HRP solution was injected into the porta hepatis and the wall of the gall bladder respectively in adult hens weighing 1.5 to 2.5 kg. After a survival period of 3 to 4 days, the hens were perfused with fixture via the left ventricle. The splanchnic ganglia, the adrenal ganglia, and the bilateral spinal ganglia and the sympathetic trunk ganglia in the thoracic and lumbosacral segments were collected and sectioned at 50um with frozen microtome. The sections were processed using 3-3 ,5-5 'tetramethylbenzidine(TMB) and examined under bright-light microscope. The result is as follows: The sensory neurons of spinal nerves innervating the liver and gall bladder were located in the spinal ganglia in the same segments T2-T7, and accumulated in the spinal ganglia in segments T5-T6 and T6 respectively. The sympathetic postganglionic neurons innervating the liver and gall bladder were located in the adrenal ganglia, the splanchnic ganglia, and the sympathetic trunk ganglia in segments T2-T7 in which the peak innervation fields were located in the segments T5-T6 and T6 respectively. The result showed that the distribution and localization of the sympathetic postganglionic neurons and the sensory neurons of spinal nerves innervating the liver and gall bladder of the fowl were similar primarily.