Application Of Microsatellite Markers To Line Identification Of White Egg-type Layer

In this study, 10 microsatellite markers were used to analyze four lines (A, B, C and D) of W-98 white egg-type chicken GP imported by Shanghai Xinyang poultry breeding center in 2002.The genetic heterozygosity, polymorphism information content (PIC), effective allele number, DA genetic distance among lines were calculated and dendrogram was constructed to evaluate the genetic variability within line and genetic relations among lines. The results were as followings:1. 6 of 10 microsatellite loci were higher diversity (PIC>0.5) except that MCW0154 locus was single in the four lines of W-98 white egg-type chicken GP, and 3 microsatellite loci were medium (0.25 B line (3.3515) > C line (2.4866) > A line (2.4830). In whole population, the matrilineal genetic diversity was abundant, and the patrilineal population consistency was higher.4. At the LEI0252 locus, the allele size of A line was from 270 bp to 290 bp, the allele size of B line was from 251 bp to 265 bp, the allele size of C and D lines was from 195 bp to 235 bp.LEI0252 locus could distinguish A line from B line, A line from C and D lines, and B line from C and D lines. 270 bp and 290 bp could be seen as the characteristic bands of A line, 251 bp and 265 bp as the characteristic bands of B lines at this locus. Therefore, the LEI0252 locus could be considered as the fine locus for line identification.5. It was found that the genetic distance of A and C lines was the biggest (0.6145), C and D lines was the minimum (0.2850) by calculated the DA genetic distance with the cluster software (Ver 1.2). The four lines were divided into 2 clusters by UPGMA, C and D lines were the first cluster, and A and B lines were the second cluster.