| The microecological health techniques on popular canker (Cytospora chrysospermd) was researched by the ways of mycology and pathology.Inhibiting effects of 3 Trichoderma strains (Trichoderma viridel, Trichoderma viridel, Trichoderma hazerium) and Chaetomium sp. on Cytospora chrysosperma were studied. The results showed that the strain Trichoderma viridel has obvious inhibited growth on Cytospora chrysosperma, the relative inhibiting effect is 11.49 for 74h antagonistic culture.The results of culture characteristic and biological characteristic of toxic mushroom strains showed: (1) The optimum growth temperature of Clitocybe dealbata, Lactarius vellereus, Amanita pachycolea, Ramaria ephemeroderma, Lepiota cristata and Amanita virosa is 25C; that of Lepiota clypelolaria and Amanita sp. is 20C. (2) The optimum carbon source of Ramaria ephemeroderma is maltose; that of Lepiota cristata is xylose, that of other strains is Glucose. (3) Except Clitocybe dealbata growing best on no-nitrogen source, the optimum nitrogen source of other strains is peptone. (4) The optimum growth pH of Amanita sp., Amanita pachycolea, Lepiota clypelolaria, Amanita virosa and Ramaria ephemeroderma is 6.0; that of Lactarius vellereus and Lepiota cristata is 7.0; that of Clitocybe dealbata is 7.5.The results of inhibiting effects of 9 toxic mushroom strains on Cytospora chrysosperma showed that the strains Amanita virosa, Ramaria ephemeroderma, Lepiota clypelolaria and Clitocybe dealbata have obviously inhibited growth on Cytospora chrysosperma. The toxin extracts of strains Amanita virosa, Clitocybe dealbata and Ramaria ephemeroderma showed significantly inhibiting effects on either mycelia growth or spores germination of Cytospora chrysosperma. Inhibiting effect (concentration is 0.5%/1%) on mycelia growth is respectively 17.3%/68.27%, 20.4%/49.73%, 16%/43.07%; diameters of inhibitory zone are 2.50cm, 1.78cm, 2.20cm; the inhibiting rates of spores germination respectively are 97.50%, 95.56%, 96.00%.
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