| Pollen of Citrus grandis var.shatinyu Hort. and Citrus grandis are chosen as experimental material. The protein abstract solution from pollen wall of shatinyu and Citrus grandis, which is abstracted in different time stages (5min.10min.20min.30min.40min.50min.60min.3h) by pH6.7 1% sodium chloride solution which contains 10% saccharose, 0.45mM Ca2+(Cacl2 ·6H2O), 1.63 mM B(H3BO3) in icebath, is dialyzed. And then the optical density of them is tested respectively by the ultraviolet spectrophotometer at 280nm spectra. As far as the method of extraction is concerned, it is an improved one just because it speeds up the experiment process. The result shows as that follows: (1) The protein content of abstract solution from pollen wall of shatinyu and Citrus grandis in 40min. increased with time. It reached its peak in 40 min and from that point on, it slowed down and maintained a certain level, as for shatinyu, of 0.364 mg/ml; Citrus grandis, 0.488 mg/ml. (2)The protein content of abstract solution from pollen wall of shatinyu and Citrus grandis in 5min. came to half of the total amount, which coincides with the study on Oenothera organensis by Lewis.(3)We make a further study on the releasing behavior of the protein from Pollen Wall of shatinyu and Citrus grandis by microsoft,and discovered that the latter is larger in quantity and quicker in speed. Moreover, we compared the protein abstract solution from pollen wall of shatinyu with that of Citrus grandis by the means of electrophoresis such as PAGE,SDS-PAGE,IEF-PAGE,IEF/SDS-PAGE. The result showed as follows: (1) By the means of PAGE in combination with Coomassie brilliant blue R-250 dyeing, the protein abstract solution from pollen wall of both shatinyu and Citrus grandis shows 7 obvious segments. Moreover there is a brand named A in segmentⅤin both of their PAGE collection of illustrative plates. But their PAGE plates are different from each other. For example, as for shatinyu, the protein abstract solution from pollen wall showed most of the total amount in 5min, and then it varied within a range that is more or less than that. As for Citrus grandis. The protein abstract solution from pollen wall did not show most of the total amount until 40 min. And the amount of that in 50min, 60min or 3h is more or less than that in 40 min., which occurs in segment Ⅲ,Ⅳ,Ⅶ, andⅤ～Ⅵ. (2)With the aid of SPSS Microsoft, compared with the protein marker under the same reaction condition, the molecular weight of the main protein from pollen wall of shatinyu is focused on section 1, 3, 4 and 5, and that of Citrus grandis , section 2,4 and 5 through SDS-PAGE. (3)We discover that the protein abstract solution from pollen wall of shatinyu and Citrus grandis both have the same protein brands in pH8.0, pH6.5～5.8, pH5.8～5.4, pH5.4～4.4 by the use of IEF-PAGE, although there is difference in the character of their protein in different time stages. Main proteins of shatinyu are alkaline and those of Citrus grandis, not only alkaline but acid. (4) By the use of mini-2-dimention electrophoresis in combination of the silver dyeing, it is found that their pattern of protein is different from each other. As for pH, the protein spots of shatinyu are focused on 7.2～5.8,that of Citrus grandis, 8.0～6.5 and 5.4～3.95. As for molecular weight,the molecular weight of the protein from pollen wall of shatinyu ranges mainly from 14.4ku to 43.0ku, and that of Citrus grandis, 20.1Ku～43.0Ku and 31.0Ku～97.4Ku. It should be noted that the little molecular weight proteins from pollen wall of shatinyu in pH7.2～5.8, such as spot a, b, c, d and e. Spot e is characteristically similar to the style S-glycoprotein associated with gametophytic self-incompatibility(pH6.2～9.5,MW 24KD～32KD). Based on two hypotheses of the self-incompatibility, such spots as a, b, c, d have a close connection with the S gene expression product of pollen. The results of experiments indicate that the similarity of protein from pollen wall of both shatinyu and Citrus grandis is reflected by their commonness...
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