| These studies have three parts. At first part, the medicine screen tests were carried through normal immersion methods out Boophilus microplus life cycle of the four different grows phase in vitro. Second part, the new compound preparations according to the best design theory of pharmaceutics and obtianing the dates of pharmacology were develped. Then, not only physical and chemistry stability of those new preparations were surveied, but also, the medical effect of those relative steadily preparations were compared. Third part, the acute and acculation toxicity of the best three-medicine effective preparations were studied. Above studies will be based massiness on the declation and entering market of new compound preparations. Their contents, methods and results in detail are following: Owing to be absent to medicine evaluations system with mass medicine. At first part, the medicine screens tests were adopted by normal immersion methods in Boophilus microplus life cycle of the four different grow phase in vitro. The results show that the botanical insecticide (Melia toosenda, Celangulins and Pyridazios) can kill and control Boophilus microplus life cycle of the four different develops periods. It will supply the theoretical authority for developing the new compound preparations. The second part, The physical stability items and mehods of the new compond preparations are including: the color of emulsions and by nude eye examing, and delaminationt variety were carried out their steroid by the low and high speed centrifugal.Their centrifugal speed and time are 4000rpm,15minï¼›12000 rpm,10min. The outburst of emulsions was observed by photics microscope. The chemistry stability items of the new compond preparations are included: pH and the emulsions kinds. PH meter measured the pH of the emulsions. The emulsions kinds were used the methods of water diluter, semblance and Cocl papers. The same contents of the emulsions were used above same methods in the common,centrifugal ,low temperature (40C),high temperatures(600C )and 15day conditions. The medical effect comparing tests of the relativly stability preparations were carried out the nenovla and engorge female ticks of Boophilus microplus in vitro again. The results show that three stablility preparations with botanical insecticides can improve highly the medicine effect in Boophilus microplus. Comparing with the Melia Toosenda, which is most low half death dose medicine in above tests, the half death dose of three new new compand preparations reduced 4.24times ,2.68times ,2.88times ï¼›1.39times ,1.01times,1.06times in the neolarval and the engorged adult female ticks separately. Comparing with the Deltamethrin, which is most common medicine at present against Boophilus microlpus, the half death dose of three new dosages reduced 12.14times,7.46times,8.02times and 9.55times,7.28times,7.62times, in the neolarval and the engorged adult female ticks,separately.The third part, the acute and cumulation toxicity of the best three-medicine effect preparations were studied. In acute and cumulation toxicity tests, give rats medicines by irrational stomach. In the results of acute toxicity tests, comparing with the Melia Toosenda,the toxicity of three new compound preparations have reduced 2.88times,1.65times,1.20times; Comparing the Deltamethrin(acute oral for rats 56.2mg/Kg), the toxicity of three new new compound preparations have reduced 50.16times,28.66times,20.79times. The tests results of acute toxicity also show that the three new compound preparations all are belonging to low toxicity medicines. The results of cumulation toxicity tests show that the cumulation toxicity Coefficient of three new dosages are above 5.0. The three new compound preparations all are belong to invisible acculmation medicine.The Safe margine and IE of the three new compound preparations are 31.22,0.2240,5.229ï¼›14.246,6.231,4.753. Above studies have improved that the physical and medical steady the new compound preparations with botanical insecticide were...
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