Study On Micro Propagation Technique Of Plant Tissue Culture Of Several Medicinal Aromatic Plants

The materials of medicinal aromatic plants Chamomilla recutita L, Mentha spicata L, Lavandula angustifolia Mill, Origanum vulgare L, Salavia officinalis L andThymus serpyllum L.var.mongolicus Ronn were used to establish the in vitro plant and develop a feasible and practicable system of, to study the effect of some phytohormones on growth, development and proliferation in vitro, to choose the suitable media of the six plants. Multiple shoots were produced from the base of buds after the establishment of the culture.Regard to Chamomilla recutita L, Mentha spicata L, Lavandula angustifolia Mill, Origanum vulgare L MS basal media is suit, but infancy Origanum vulgare L grew better on Nitsch medium than MS medium. To Thymus serpyllum L.var.mongolicus Ronn of growth, MS medium is better than amend MS medium.Regard to Chamomilla recutita L, multiplication and growth of shoots were optimum with MS+ BA0.2mg/L+IBA0.06mg/L. MS medium with NAA(0.1~1.5mg/L) or IBA(0.1-1.5mg/L) can induced lots of roots, the survival rate after transplantation was higher. cuttings were stronger on MS medium with BA×IBA than MS media with BA or MS media with IBA.Regard to Mentha spicata L, multiplication and growth of shoots were optimum with MS+BA0~0.1mg/L+IBA0~0.04mg/L. plant of regene ration are easy to root. The ratio of rooting can reach 100% in MS+ NAA(0.1~0.5mg/L).And plants produce many aerial roots. And cuttage of Mentha spicata L out of bottles were studied . Regard to Lavandula angustifolia Mill, the optimum propagation medium is MS+BA1.0mg/L; the optium-rooting medium is MS+IBA0.5mg/L. The survival rate after transplantation was not high. Growth of stem-tip is better than stem.Regard to Origanum vulgare L, shoot-tips as explants, the optimum propagation medium is MS+2.0mg/LBA+IBA0.02mg/L. The ratio of rooting can reach 100% in MS and 1/2MS media, but a number of roots are thinner and fewer, and do not survive after transplant. So it is necessary that hormone should be added in medium. Regard to Salavia officinalis L, the optimum propagation medium is MS+BA0.8mg/L.The effect of BA,IBA on bud multiplication is significant. The best rooting medium is optimum with 1/2MS+IBA0.8mg/L+ sucrose 20g/L.Regard to Thymus serpyllum L, the optimum propagation medium is MS+BA1.5mg/L+IBA0.08mg/L, the optimum-rooting medium is 1/2MS+IBA0.5mg/L+ sucrose 20g/L.The ratio of rooting can reach 100%.