The Artificial Insemination And Sexual Molecular Identification Of Reindeer

In China reindeer immigrated Daxinganling with Ewenkers together in order to avoid chaos of war three hundred years ago. Reindeer formed their special living habits under local environment after hundreds of isolated rearing, which were rare species in south. Research on artificial rearing reindeer had never been carried out in China. Now hunters in Ewenke had been rearing reindeer by semi-wild rearing way that is traditional extensive management. Reindeer searched for food freely around temporarily residential area without being watched and supplementary feed. Reindeer breed by natural mating, and which were eliminated through nature, close relative mating declined in reindeer community seriously, which limited the development of cultivating reindeer. The application of artificial insemination technique of reindeer improved the quality and availability of breeding reindeer, and disseminated fine genes most extensively, and avoided spreading disease and loss due to fighting of breeding male reindeer. Reindeer were only one kind of deer whatever it was male or female had antler. Reindeer were considered fake thing in the traditional Chinese medicine. Now the ways of determining that it was male or female mainly included properties determine, determine while being micro-, the physics and chemistry determine and so on, molecular determine was never researched. The research of molecular determining male and female antler perfected differentiating reagent box of fake antler.Reindeer were breed under isolation in China, reindeer community declined seriously. The research of genetic diversification of reindeer provided molecular base for breeding of reindeer in China, and provided possibility for the future molecular breeding research, and provided basis material for the research of community heredity, heredity differentiation and systematic growth.The experiment was divided into three parts. First, artificial insemination of reindeer was carried out. Primary research results of artificial insemination of 11 reindeer show that Estrus Synchronization and artificial insemination were ideal, Estrus Synchronization and artificial insemination rate were 100%. Secondly, the molecular biology determine of reindeer was carried out. Adopting the molecular biology qualification method in the research, SRY genes of male and female antler were increased, we could see from increased picture prime adopted could differentiate female antler and male antler accurately. We use DNAMAN4.0 to structure the tree among Reindeer and Sika deer and Red deer.We can draw a conlusion that The similarity of Sika and Red deer are 0.99,that the similiraty among Reindeer,Sika deer and Red deer are 0.37.Thirdly, genetic diversification of reindeer was researched. 24 primes were increased by RAPD-PCR, from which 14 increased clear primes were sifted. I analyse the kindred of these 13 Reindeers with DNAMAN4.0,and draw a conclusion that the similiraty is between 0.584 and 1.000,that the similitude coefficient of 14 primes are 0.792. Li Haitao Animal Genetics and Breeding Prof. Bai Xiujuan...