Studies On Hormonal Levels And Development Of Ovary And Uterine Gland For JiangQuHai Gilts

The present study was conducted to investigate the development of ovary and uterine gland, followed by the change of circulating E2 and P, and ESR mRNA expression in ovary and uterus from birth to Postnatal Day(PND)15, 30, 45, 60 for JiangQuHai gilts. The main results are as follows:1. The same sections of ovary and uterus are collected from birth to PND 15 , 30, 45 , 60 and made paraffin slices, and then made pictures via dyeing .From ovarian tissue slices, we can find that ovarian cortex sections have large numbers of primordial follicles, and tabular granulosa cell rank at the surrounding of the oocytes. With the age increasing, primary , secondary follicles gradually appear, and granulose cells become thicker, the volume of follicles become bigger. For uterus, we find that endometrial gland of neonatal piglets is little in the volume, synchronously small in quantity, and stroma is also few. Between PNDs 15 and 60, the endometrial gland continuously develops and proliferates and become bigger gradually in the volume, and more and more in quantity. At the same time, the stroma becomes denser.2. Blood samples are collected from birth to PND 15 , 30 , 45 , 60. Concentrations of E2 and P in serum are measured previously by radioimmunoassay, and evaluate their changes. The hormonal concentrations of E2 and P are high at birth, but decrease rapidly after birth and keep low levels before puberty though have a rising trend from PNDs 30 to 60. Between PND 15 and 60 ,the Pearson correlation is 0.819(p<0.01) between serumal E2 and postnatal day; and is -0.739 (p<0.05) betweenserumal E2 and P.3. Estrogen receptor mRNA expression abundance in ovary and uterus are investigated by in stiu hybridization histochemistry (ISHH).Results of image manipulation analysis system indicated that ESR mRNA almost are not detected at birth in the ovary; ESR mRNA expression increased from PNDs 15 to 45, and declined slightly on PNDs 60; Positive staining was mainly detected in primordial follicles, primary follicles and secondary follicles, thicker in big follicles than in small follicles; In uterus, positive intensity of ESR mRNA had the significant correlations with PNDs, and ESR mRNA was mainly detected in endometrial gland and lesser in stroma. The ESR mRNA expreesion in uterus is not dependent on ovary.4. In conclusion, the present study first uses the way of ISHH to investigate ESR mRNA expression abundance in ovary and uterus of JiangQuHai gilts and establish an approach to evaluate the total levels of ESR mRNA. On the other hand, the study explains their relativities between morphopoiesis of reproductive tissues and sex hormonal concentration in serum and ESR mRNA expression abundance prepuberty for JiangQuHai gilts. At the same time, the present study can offer the valuable materials to develop a new strain of swine, and establish the basis of exploring the relations between the difference of ESR transcription , translation levels and litter size.