Studies Of Tissue Culture Of Virus-free Plant

Three kinds of virus-free plants about Chrysanthemum, Tulip and Caryophyllus were studied in the paper. In test of virus-free Chrysanthemum plant, the Chrysanthemum stem tips with a length of 0.5-0.8mm is suitable for explants, which came from "Guoqing" Chrysanthemum with CMV. They were inoculated in MS medium with different consistency of BA and NAA. The results showed that the proportion of BA and NAA affected callus inducing and growing. Callus grew well on MS+BA2.0mg/l+NAA0.1mg/l, 96.0% explants formed. Callus differentiation was affected by different consistency of BA and NAA. About 60.0% callus developed on MS+BA3.0mg/l+NAA0.1mg/l, and there were 4 buds every callus. The buds were cut and transferred into 1/2MS medium, and they generated small write roots after 10 days. The rooting rate was 78.0% into 1/2 MS medium without NAA, and more than 92.0% into medoum with different consistency of NAA. But it was good to rooting into medium with l/2MS+NAA0.1mg/l. In test of virus-free Tulip plant, stem tips with a length of 0.3-0.5mm is suitable for explants. Stem tips induced callus well on medium MS+NAA2.0mg/l+BA1.5mg/l, with 94.0% explants formed. About 86.0% callus of Tulip developed on MS+NAA0.5mg/l+BA 1.5mg/l. About 60.0% Tulip stem tips directly diffrented into buds well on MS+NAA0.1mg/l +2,4-D0.1mg/l. The rooting rate was 80.0% into l/2MS+NAA0.5mg/l, and there were 5.2 buds every callus. In test of virus-free Caryophyllus plant, stem tips with a length of 0.3 mm is suitable for explants. The Caryophyllus callus grew well on MS+BA0.7mg/l+NAA0.1-0.2mg/l, 90.0% explant formed. About 74.0% callus developed on MS+BA0.8mg/l +NAA0.2mg/l, and there were 5.9 buds every callus. The rate of rooting and transpiant were 86.0%-94.0% on l/2MS+NAA0.01mg/l+IBA0.05-1.0mg/l. The ratio of Caryophyllus glassy plant counld be lightened through reducing culture tempurature, increasing illumination or adding penicillin into medium. Virus-free plants of three kinds of flower were tested by sap transmission, symptom observing and endosome dyring. By testing, there were 19 Chrysanthemum virus-free plants, which virus-free rate is 63.3%, 13 Tulip virus-free plants and Caryophyllus virus-free plants, which virus-free rate is 65% in the paper.