Studies On Induction And Mechanism Of Apoptosis In Rice Suspension Cell

To inspect signal transmission and mechanism of plant apoptosis, it is necessary to establish an effective and stable induction apoptotosis system in plant cell. Because the system can provide us an effective and reliable way to select plant elicitors and a new path to elect elite varieties of high resistance and anti-adversity for crop breeding. This paper established the induction apoptotosis system of rice suspension cell and discussed the induction apoptotosis mechanism. The results as follows:Different rice suspension cell systems were established using callus cell of different rice breeds and germintions and the growth status of different suspension cells were compared. The different extraceller POD producing abilities were discussed. The results indicated that rice suspension cell can be established easier when rice callus cell was better dispersive and more difficult to brown. Along with germintion of Zaoxingl06 callus cell, activity of extracelluar POD and suspensibility (A600) raised at first and then declined in suspension cells. In same germintion of callus cell, extracelluar POD/A600 of Weiyou46 and Jingdaol suspension cell were higher, comparing to Zaoxingl06 and Zhefu802.The activity of extracelluar POD induced by exogenous H2O2, CaCl2 and SA were different from each other , respectively. After Zaoxingl06 suspension cell was treated with 3mmol/L H2O2 , activity of all extracelluar POD was inhibited. But activity of extracelluar POD was increased by high concentration of exogenous SA and CaCl2, and new POD isoenzyme was not produced by PAGE. While the changes of extracelluar POD activity was not obvious when Peiliangyou288 suspension cell was treated with 3mmol/L H2O2.After Zaoxing106 suspension cell was treated with 3mmol/L SA, H2O2 and CaCl2 for 48 hours, its DNA became fragment at some degree in all treatment. But DNA fragmentation in Peiliangyou288 suspension cell was found only by treatment of 3mmol/L SA. Some changes of apoptosis cell such as the cytoplasm concentrating, chromatin aglomeration and nucleolus disjoint were also observed using the DAPI fluorescence dyes.After Zaoxing106 suspension cell was treated with SA, the activity of celluar catalase was dramatically inhibited(P<0.01) and soluble protein content was dramatically decreased(P<0.01),but the activity of celluar catalase and soluble protein content were both increased after treated with H202 and CaCl2. After Zaoxingl06 suspension cell was treated with SA, H2O2and CaCl2 the activities of celluar POD were all inhibited, but after treated with H2O2 the inhibition was the most remarkable. After Peiliangyou288 suspension cell was treated with SA, the activity of celluar catalase was dramatically inhibited (P<0.01) and soluble protein content was dramatically decreased (P<0.01),but the activity of celluar catalase and soluble protein content were both increased after treated with H2O2 and CaCl2. After Peiliangyou288 suspension cell was treated with SA, H2O2 and CaCl2 the activities of celluar POD were increased at different degree, respectively. After rice suspension cell was treated with signal molecules, the activity of celluar catalase and soluble protein content were highly positive correlated(r=0. 92478).