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Analyzed Relationship Between CLPG, BMP15 Gene Polymorphic And Production Performance Of Seven Sheep Populations In North Of Xinjiang

Posted on:2005-03-06Degree:MasterType:Thesis
Country:ChinaCandidate:R DaiFull Text:PDF
GTID:2133360122993093Subject:Animal breeding and genetics and breeding
Abstract/Summary:PDF Full Text Request
The body sizes data (body weight, body high, body length, heart girth, chest width, chest depth and circumference of cannon bone) of Chinese Merino lamb before being weaned were analyzed with the methods of correlations and regressions. The result showed that the single correlation between the characters of weight and heart girth, chest width and circumference of cannon bone was significantly different. The correlation between weight and heart girth, chest width, circumference of cannon bone was highly positive correlation. Among seven regression models, the correlation index of six regression models was over 0.7, but that of 60 days' was 0.452. The result suggested that selecting body weight was efficient through heart girth, body high, chest width and circumference of cannon bone.The genetic variation (gene frequency, heterozygosity, polymorphic information content(PIC) and effective number of alleles) of 3 microsatellite loci(IDVGA30, BMS1516, OY3) was analyzed in 6 sheep populations (Chinese Merino, Suffolk, Dorset, FI of Suffolk crossed with Xinjiang Fine Wool, F] of Doset crossed with Xinjiang Fine Wool and Charolais sheep). The result showed that PIC, heterozygosity and effective number of alleles of 3 microsatellite loci ranged from 0.5253 to 0.7243, from 0.6425 ti 0.7682, from 2.1624 to 4.4705, respectively. The clustering dendrogram from genetic distance of the three microsatellites could correctly reflect the geographical distribution of and the relationship within the six sheep populations. The genetic distances of Chinese Merino between foreign sheep breeds and cultivated breeds bearing foreign bloodline were relatively large. Consequently, they clustered in two groups. The phylogenetic relationship between different sheep breeds was in accordance with their resource, breeding history, differentiation and locations.The relationship between different genetype of 3 microsatellites and body sizes in Chinese Merino lamb was analyzed. The mean weight of genetype AB(19.73kg) was the highest and the mean body length of genetype DD(56.00cm ) was the longest in microsatellite IDVGA30 in 90 days old. In microsatellite BMS1516, the chest high of genetype EF(22.44cm), the heart girth (62.28cm)and the circumference of cannon bone (8.07cm) of genetype CD were the highest in Chinese Merino lamb of 90 days old. In microsatellite OY3, the born weight of genetype EF was the highest(4.10kg).Tow SNP locus of CLPG gene were analyzed through the method of PCR-RFLP and PCR-SSCP. They all showed polymorphism of SSCP. The mutatant genetype wasnot detected through the Forced-RFLP when analyzing the gene BMPl5.
Keywords/Search Tags:Sheep, CLPG, BMP15, Polymorphic
PDF Full Text Request
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