Studies On The Inheritance And Molecular Makers Of Aphis Sacchari. Zehntner Resistance In Sorghum

Aphis sacchari. Zehntner was one of important pests in sorghum. This leaded to serious loss of yields and quality in sorghum of the world. Breeding resistance cultivars was the most economical, preferable, and effective method to control the Aphis sacchari. Zehntner. In the near years, development melocular marker technology fasten the progress of crop breeding. Moreover, the study of molecular biology of Aphis sacchari. Zehntner in sorghum lagged to other crops and further affected the progress for breeding Aphis sacchari. Zehntner resistant varieties.The research on the identification method resistant inheritance and SSR molecular markers of Aphis sacchari. Zehntner was carried out by using the cross of He nong 16xQian san.The intention was to explore a identification method of Aphis sacchari. Zehntner resistant, understand the characteristics of resistant inheritance of Aphis sacchari. Zehntner, establish suited SSR technique system and search the molecular marker linked with the resistant gene. It would build a basis for mastering regulation of Aphis sacchari. Zehntner resistant inheritance and cloning the resistant gene. The main results as follows:1 Identification and genetic analysis of resistance of Aphis sacchari. Zehntner by parents F1 and F2 population of He nong 16xQian san indicated that He nong 16 was immune to Aphis sacchari. Zehntner, Qian san was impressible, Fi were high resistant to Aphis sacchari. Zehntner, the segregation rate of resistant and susceptible was 3:1 in F2 population. So this resistance were controlled by one gene, Fi showed complete dominance.2 He nong 16 which was a hard-won variety immuned to Aphis sacchari. Zehntner was used in this expirement. Single factor selection was made to investigate better concentration in SSR technique system.Then, random thorough experiment was made to seek the best combination among factors.At last, the best SSR technique system was established. Taq DNA polymerase 0.3uL(5u /uL ), 10XPCR Buffer 2.0uL, primer 2.0uL (10umol/L), template DNA 60 ng.Using this system, amplification bands were clear, steady and non-especial bands were shorter.3, 99 pairs of SSR primers were amplified among DNA of P1 P2 and theirsegregated bulk of the cross of He nong 16xQian san. The results indicated that 17 pairs of SSR primers had visible and steady polymorphism between parents, distributed in 8 linkages, the rate of polymorphism was 17.8%. One pair of SSR primers had polymorphism between gene pools. It indicated the inheritance background of He nong 16 was very different from Qian san. So the higher genetic diversity in population ,the better for construct a linkage map. 4 The PCR amplification products of F2 recessive individuals from the cross He nong 16xQian san by 17 pairs of primers were analyzed. There was one SSR loci ?Xtxp6 related to the susceptible genes of Aphis sacchari. Zehntner. The PCR amplification products of all of F2 individuals were analyzed by using MAPMAKER soft program. It was assured elementarily that Aphis sacchari. Zehntner resistant gene linked to Xtxp6. It located at zero loci of 9 linkage, with a distance of 11.7 cM.The innovation of this research was that He nong 16 which was a hard-won variety immuned to Aphis sacchari. Zehntner was used, single factor selection and random thorough experiment were made to establish a suited SSR technique system, SSR molecular marking technology was introduced to find genes relevant to Aphis sacchari. Zehntner resistance. This would build a basis for isolating and cloning the Aphis sacchari. Zehntner resistant genes in the future.