Studies In The Vitro Culture Of Strawberry

1 .We treat the three breeds of strawberry,Akihime , Toyonoka, Tochiotome,as the test samples to study the vitro culture of strawberry.On the basis of MS media,we test the three breeds in a variety of conditions during the process of leaf regeneration:to add different couples of growth hormone B A/IB A (0/0.1,2.0/0.1,4.0/0.1,5.0/0.1,6.0/0.1 mg.l-1) BA/TDZ(0.1/0,0.1/0.5,0.1/1.0,0.1/1.5 mg.l-1),at different age(12-16d,16-20d,20-28d,28-35d),in dark for several days(4d,6d,8d,10d),to add AgNO3 and by different organs.lt showed that the regeneration ability of Akihime finishs the first and that of Tochiotome takes the second plase,Toyonoka takes the last, when we added BA/IBA (5.0/0.1 mg.l-1)or IBA/TDZ (1.0/1.0 mg.l-1), it had better regeneration ability. When the leaf of Akihime and Toyonoka were at the age of 20 to 30 days and that of Tochiotome was 30 to 40 days old, their adventitious buds had the greatest inductivity. When added BA/IBA and set in dark for 6 days or added TDZ/IBA or BA2,4-D ,set in dark for 8 days, the regeneration achieved best results. AgNO3 could promote the regeneration of lamina's adventitious bud. The lamina regeneration ability of Akihime and Toyonoka were better than that of their leafstalk. While culturing the stem-tip on the basis of MS medium, adding BA/NAA of different concentration, we found that when the concentration was 1.0/0.01 mg.l-1,it produced the best effect.2. We treat the three breeds of strawberry,Akihime , Toyonoka, Tochiotome,as the test samples to study the effect of PP333 on the preservation of the tube-seeding at normal atmospheric temperature. In the MS medium of different paclobutrazol(PP333) concentrations(0,0.4,0.8,1.2,1.6,2.0 mg.l-1) ,we had cultured three different breeds of strawberry(Akihime, Toyonoka, Tochiotome) for four months. It showed that when the PP333 concentration was 1.2-1.6 mg.l-1, the antiblastic effect on Akihime is of the best,while when the PP333 concentration was 0.8-1.2 mg.l-1, that on Toyonoka and Tochiotome are of the best. And higher pp333 concentration promoted the rootage.3.We use the test sample of Tochiotome to study the vitrification in the vitro culture of strawberry, After culturing the seedling of Tochiotome on the MS medium under the circumstance of different carbon sources (saccharose,fructose and dectrose are 30mg/l individually),different agaragar concentrations (4,6,7,8 mg.l-1), different BA concentrations(2.0,4.0,5.0,6.0 mg.l-1) and different ventilation (to seal the bottle with polypropylene plastic film or with brown paper and polypropylene plastic film), we could conclude when the agaragar concentration was 7 mg.l-1, it helped decrease the vitrification of the seedling. It was not obvious for the BA concentation and ventilation to reduce the vitrification of the seedling, so it still need further observation.