| Activities of 4.0% NJ-1 EC, which is a kind of sulfocynate chemicals, and its mixture with avermectin were studied. Results showed that both NJ-1 and its mixture with avermectin had a strong lethal effect on J2 of Meloidogyne incognita. NJ-1 killed J2 with a LD50 4.6269 g/mL and LD90 5.7437 g/mL. Rate of hatch of eggs was reduced 99% while treated with NJ-1 dilution of 16ug a.i./mL and 8ug a.i./mL for 48h. Dilution of 5.3,4, 2.3, 2 g a.i./mL also inhibited over 92 percent of hatching 48h later. At the same time majority of J2 were dead. 4.0% NJ-1 EC was mixtured with avermectin at the proportion of 40:1, 40:2 and 40:3, and CTC was 117.2, 129.8 and 161.0. While the proportion of avermectin was increased, synergism was raised.Phytoxicity of 4.0% NJ-1 on vegetable growth was tested for transplanting seedlings of cucumber, potato and capsium. These vegetables were transplanted to soils pre-treated with NJ-1, or were treated by root-pouring after transplanting in greenhouse. Also cucumber was tested in field by the same way. Neither soil treatments on the seventh day before transplant with 50, 40 and 26.7 g a.i./mL dilution, 50mL per pot, nor root-pouring seedling planted with 40, 20, 13.3 and 10 g a.i./mL dilution, 30mL per pot in greenhouse, it was no harm to cucumber. But all the above dosage tested of NJ-1 were harmful to tomato and capsicum. In field, soil was treated at 6.25L/m2 of dilution with 50,40, 26.67 g a.i./mL on the seventh day before, or root-pouring at 2.5 L/m2 of dilution with 20ug a.i./mL just after transplanting. None damage was found on cucumber during all the breeding season. So, NJ-1 is safe to cucumber.Optimal treatment method, opportunity and dosage were determined. NJ-1 was used to control cucumber root-knot nematode by two ways. One was soil treatments before transplant, another was root-pouring treatments when seedlings transplanted at different concentrations. The control efficacy was copared. Results suggested the further was better than the later. Control efficacy was 50.0%, 74.0% and 61.0% for three, four and seven months respectively, while soil treatment at the dosage of 6.25L/m2 of 50 ga.i/mL dilution. It was better than that of avermectin. Root-pouring treatment while seedlings transplanted also expressed satisfied control efficiency until three months later.According to the different cultivation system, the treatment method was further optimised. Compared with the last year, this method reduced forty percent of NJ-1 with corresponding control efficiency, and the yield was obviously accelerated. On the basis of soil treatments before transplant, control efficiency was raised by one more application by root-pouring treatment during the breeding with NJ-4 diluents of 2500 folds.Forty-one single-spore isolates causing Capsium Anthracnose collected from Jiangsu and Hainan Provinces were identified into Colletotrichum gloeosporioides and Colletotrichum capsica according to conidial morphology. Of these isolates, 58.54% was C. gloeosporioides. Sensitivities of C. gloeosporioides and C capsica isolates from capsium to azoxystrobin were determined by spore germination test oh slide. EC50 values of azoxystrobin inhibiting spore growth of these tested isolates ranged from 0.00028 g/mL to 24.987 g/mL .The mean EC50 values for 24 isolates of C. gloeosporioides and 17 isolates of C. capsica isolates were 5.2336 g/mL and 2.9655 g/mL respectively. Result showed that C. capsica was sensitiver to azoxystrobin than C. Gloeosporioides. |
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