| The fragment of tpsl was digested by two restriction enzyme Kpnl/Xbal ,and then was successfully joined with Pwml01 plasmid which was digested by Kpnl/Xbal too.The outcome was expression vector Pwml02,which wastransformed into Agrobacterium tumefaciens EHA105.By changing the concentration of the 2,4-D,we get a good inducing and differentiation medias, and deciding using the mature and immature embryos of Wen 6 as explants.Agrobacteriurn mediates transforming the TPS1 gene into the callus forming by mature and immature embryos of wen 6.Then we get some resistencing callus which could growing on medias containing 20-60mg/1 hygromycin.We get some regernerating wheats and four of them are positive by PCR analysis.These four plants were indentified to bethe transgenic wheats. |
