Comparison Among Four Lines Of A.M.ligustica As Genetic Resource

Through morphological markers, MDHâ…¡ isoenzyme markers and RAPDmolecular marker of four lines in A.m.ligusica, as genetic resources were comparedand analyzed.The results showed as follows:1 There existed significant differences (P<0.05) among adult workers of 4 lines in tongue length and right forewing area. Australia-Italian bee workers were the longest tongue and Ben-Yi bee workers the shortest. The right forewing area of Pinghu Jiang bee workers were the longest and Ben-Yi workers the smallest,And there existed no significant difference among bee workers of 4 lines in head weight. The results were different from the production capability corresponding to the four lines and demonstrated that individual morphological signs might not be regarded as a standard to evaluate population production. 2 The analysis, conducted by means of IEF PAGE, produce the flowing results iscc genotype had the highest genotypic frequency, Pinghu Jiang bee workers had the highest genotypic frequency ( ep=0.5425 ) , American-Italian bee workers had the lowest one. in gene frequency, c gene prevailed and Pinghu Jiang bee workers had the highest one, Australia-Italian bee workers the lowest.The purity degree of all lines surpassed 0.50, and Pinghu Jiang bee workers got a highest value of 0.82. The independence test indicated that differences between the 4 lines in genotypic frequency, gene frequency and hybrid-purity degree were statistically significant(P<0.01). The results demonstrated that c gene frequency might be regarded as an evaluating marker and c gene frequency might be adopted as a standard to evaluate the production of royal jelly.3 RAPD-PCR analysis of 30 random primers indicate that primer W18 (5'-CGGACGGCGG -3') and W23 ( 5'-GTACCGCCCG-3') leaded to polymorphie bands after amplification. 2353bp and 392bp all of W18 amplified bands were only in American-Italian bee workers, 2092bp band for high productivity of honey. 632bp was specific for high productivity of royal jelly. Of all W23 amplified bands, 651bp may be served as a specific band to distinguish Australia-Italian bee from other breeds. The-test results indicated that of gene frequencies polymorphic bands were significantly different among these four lines(P<0.01).4 The chaos -analysis by amplification using W18 and W23 primers showed that Pinghu Jiang bee and Ben-Yi bee might have a close kinship.