Effect Of Enzyme Supplementation On Metabolite Of Nutriments And Energy In Double-low Rapeseed Meal

This study was a part of the Project of Industrialization of Dowble-low Rape, reasonable utilization of double-low rapeseed meal in poultry diet managed by Hubei Government of the People's Republic of China. The objective of the study was to investigate the efficacy of phytase and nonstarch polysaccharidase (including xylanase, beta -glucanase, pectic enzyme and cellulase) supplementation to double-low rapeseed meal on the nutriment and energy metabolite. The appropriate level of enzyme supplementation of double-low rapeseed meal for poultry was also explored.Experiment 1. The nutriment, energy and antinutriment in meal from four cultivars double-low rapeseed (Hua Double Low 3 rapeseed, Hua Hybride 3 rapeseed, Hua Hybride 4 rapeseed, ordinary rapeseed) were analysed. The results showed that the nutriment content and energy value of the four native double-low rapeseed meal were quite similar. The results also indicated that the native double-low rapeseed meal was similar to canola meal and general rapeseed meal in its nutriment and energy, except its crude protein was slightly high. The four native double low rapeseed meal ,canola meal and ordinary rapeseed meal contained similar content of phytic acid, tannin and sinapine. Glucosinolate(GL), oxazolidine (OZT) and isothiocyanate (ITC) contents in four native double low rapeseed meal were higher than in canola meal and lower than in ordinary rapeseed meal.Experiment 2. Refering to Sibbld's TME(True Metabolitic Energy), the experiment was conducted with thirty Avian roosters allotted to ten groups at random with three birds in each group. The results indicated that the inclusion of phytase and nonstarch polysaccharidase(including xylanase, 3 -glucanase, pectic enzyme and cellulase) at the level of 1.5× 104μmol/min.g, 560μmol/min.g, 228.9μmol/min.g, 0.27 × 104μmol/min.g, 1 × 103U , respectively, could improve the nutriments and energy metabolite in double-low rapeseed meal significantly (P<0.05). The increasing level of phytase and nonstarch polysaccharidase supplementation increased the metabolite of thenutrients and energy in double low rapeseed meal subsequently. Especially, as the increasing content of phyatase the metabolite improvement was significantly different(p<0.05). The optium metabolite of the nutriments and energy in double-low rapeseed meal was observed with phytase and nonstarch polysaccharidase (including xylanase, P -glucanase, pectic enzyme) supplementation at the level of 9 × 104μmol/min.g 2240μmol/min, 915.6μmol/min.g, 0.75×104 μmol/min.g respectively. It can be concluded from all of the above that the content of nutriment, energy and antinutriment composition of native double-low rapeseed meal is similar to canola meal except that the content of glucosinolate is relatively higher. One of the major problems that limit the utilization of double-low rapeseed meal in poultry diet is its low energy value resulting in its hige fiber content and the low digestibility of crude protein and lysine. However supplementation of phytase and nonstarch polysaccharidase to double-low rapeseed meal can greatly improve the metabolite of its nutriments and energy.