The Study Of Cultural Condition And Enriching Calcium And Iodine Of The Mycelia Of Wolfiporia Cocos

Being the one of traditional Chinese medicine, Wolfiporia cocos is a kind of edible and medicinal fungi which has great value to study. When the mycelia secreted melanin, the growth of mycelia was inhibited. This paper studied the condition of solid cultivation and liquid fermentation; And this paper studied the condition of calcium-enriched and iodine-enriched of Wolfiporia cocos. The results of the study were as follows:1. Nutrition tests of Wolfiporia cocos suggested that the optimal carbon source, nitrogen source, mineral, growth factor, pH, C/N value were pinewood and glucose, corn plasm, KH2PO4and MgSO4, VB1, 3.17-6.05, 25:1-35:1 respectively. And secreting melanin of the mycelia did not relate to carbon source, mineral, growth factor, and C/N. When nitrogen source were organic nitrogen which didn't include corn plasm, the concentration of KI was high, or pH was greater than 7.0, the mycelia of Wolfiporia cocos secreted melanin.2. Temperature test suggested that when the Wolfiporia cocos which cultured 22℃ was preserved after 7 days, and which cultured 28# was preserved after 9 days, the mycelia began to secret melanin. But the mycelia which cultured 25# only began to secret melanin after 30 days.3. The strain of Wolfiporia cocos isolated from pultaceous sclerotium secreted the more melanin than that of the strain isolated from rigid sclerotium. Culturing mycelia's tip of Wolfiporia cocos could not inhibit that the mycelia secreted melanin well.4. Inoculation number and medium volume tests suggested that the optimal inoculation number was 10%, and the optimal medium volume was 50mL/150mL with Erlenmeyer flask. Through orthogonal test, the optimal medium was selected and it contained glucose 25g, corn plasm 10mL, KH2PO4 1.5g, MgSO4 0.75g, VBi 5mg, distillation water 1000mL. And this medium was better than corn plasm medium to Wolfiporia cocos. Under the optimal fermentation condition we got the variation curves of mycelia biomass, pH value, extracellular polysaccharide and endocellular polysaccharide content.5. The test of enduring calcium in the solid medium suggested that the mycelia growth was not inhibited when the concentration of CaCl2 was 2.00g/L. When the concentration of CaCl2 was 4.00g/L or greater than it, the mycelia growth was inhibited. When the concentration of CaCl2 was 2.00g/L and added with concentration grads, the test of calcium-enriched in the course of fermentation suggested that enriching ratio was 78.46%, calcium content of the mycelia was 94.25mg/g, and 26.83% was organic calcium.6. The test of enduring iodine suggested that the mycelia growth was not inhibitedwhen the concentration of KI was 0.25g/L, when it was 0.50g/L the mycelia growth was inhibited, when it was 1 .00g/L or greater ,the mycelia growth was bad inhibited. When the concentration of KI was 0.50g/L, the test of iodine-enriched suggested that enriching ratio was 1.64%, iodine content of the mycelia was 2.3mg/g, adding KI with concentration grads.